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Mini Samples ELISA Kit for Glucuronidase Beta (GUSb)
GUS-B; MPS7; Beta-G1
- Product No.MEB416Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range1.56-100ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.67ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 559
US$ 798
US$ 3591
US$ 6783
US$ 55860
For more details, please contact local distributors!
Specificity
This assay has high sensitivity and excellent specificity for detection of Mini Samples Glucuronidase Beta (GUSb).
No significant cross-reactivity or interference between Mini Samples Glucuronidase Beta (GUSb) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Mini Samples Glucuronidase Beta (GUSb) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Glucuronidase Beta (GUSb) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 95-102 | 99 |
| EDTA plasma(n=5) | 80-105 | 99 |
| heparin plasma(n=5) | 84-101 | 88 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Glucuronidase Beta (GUSb) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Glucuronidase Beta (GUSb) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Glucuronidase Beta (GUSb) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 83-101% | 94-102% | 85-99% | 81-99% |
| EDTA plasma(n=5) | 96-104% | 83-90% | 84-104% | 94-101% |
| heparin plasma(n=5) | 78-95% | 80-102% | 89-97% | 94-103% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×60µL | Assay Diluent A | 1×6mL |
| Detection Reagent B | 1×60µL | Assay Diluent B | 1×6mL |
| TMB Substrate | 1×4.5mL | Stop Solution | 1×3mL |
| Wash Buffer (30 × concentrate) | 1×10mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.
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| Magazine | Citations |
| Immunity, Inflammation and Disease | Glucuronides of phytoestrogen flavonoid enhance macrophage function via conversion to aglycones by β-glucuronidase in macrophages. pubmed:28480538 |
| European Journal of Pharmacology | Darunavir alleviates irinotecan-induced intestinal toxicity in Vivo Pubmed:30053408 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPB416Hu01 | Recombinant Glucuronidase Beta (GUSb) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB416Hu01 | Polyclonal Antibody to Glucuronidase Beta (GUSb) | WB,IHC,ICC/IF |
| LAB416Hu71 | Biotin-Linked Polyclonal Antibody to Glucuronidase Beta (GUSb) | WB; IHC; ICC. |
| MAB416Hu22 | Monoclonal Antibody to Glucuronidase Beta (GUSb) | WB; IHC; ICC; IP. |
| MEB416Hu | Mini Samples ELISA Kit for Glucuronidase Beta (GUSb) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEB416Hu | ELISA Kit for Glucuronidase Beta (GUSb) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB416Hu | Multiplex Assay Kit for Glucuronidase Beta (GUSb) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |