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Mini Samples ELISA Kit for Interleukin 1 Family, Member 9 (IL1F9)
IL-1F9; IL36g; IL-1H1; IL-1RP2; IL1E; IL1H1; IL1RP2; Interleukin 36, Gamma; Interleukin-1 Homolog 1; Interleukin-1 epsilon; Interleukin 1-Related Protein 2
- Product No.MEL621Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample Typen/a
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Rangen/a
- Sensitivityn/a
- Downloadn/a
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 665
US$ 950
US$ 4275
US$ 8075
US$ 66500
For more details, please contact local distributors!
Specificity
This assay has high sensitivity and excellent specificity for detection of Mini Samples Interleukin 1 Family, Member 9 (IL1F9).
No significant cross-reactivity or interference between Mini Samples Interleukin 1 Family, Member 9 (IL1F9) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Mini Samples Interleukin 1 Family, Member 9 (IL1F9) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Interleukin 1 Family, Member 9 (IL1F9) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 80-89 | 84 |
| EDTA plasma(n=5) | 94-103 | 98 |
| heparin plasma(n=5) | 99-105 | 102 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Interleukin 1 Family, Member 9 (IL1F9) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Interleukin 1 Family, Member 9 (IL1F9) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Interleukin 1 Family, Member 9 (IL1F9) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 79-94% | 82-89% | 81-89% | 92-99% |
| EDTA plasma(n=5) | 88-95% | 92-101% | 82-98% | 78-99% |
| heparin plasma(n=5) | 78-92% | 98-105% | 96-104% | 81-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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| Magazine | Citations |
| PLOS ONE | Interleukin (IL)-1β Is a Strong Inducer of IL-36γ Expression in Human Colonic Myofibroblasts PubMed: 26562662 |
| Journal of Investigative Dermatology | Increased interleukin‐36γ expression in skin and sera of patients with atopic dermatitis and mycosis fungoides/Sézary syndrome Pubmed:29314201 |
| Journal of Investigative Dermatology | For Review Only |
| international immunopharmacology | Acitretin inhibits IL-17A-induced IL-36 expression in keratinocytes by down-regulating IκBζ Pubmed: 31863918 |
| J Agric Food Chem | Inhibition of HMGB1/TLR4 Signaling Pathway by Digitoflavone: A Potential Therapeutic Role in Alcohol-Associated Liver Disease Pubmed:35212223 |
| Br J Pharmacol | Modulation of IL‐36‐based inflammatory feedback loop through hepatocytes‐derived IL‐36R‐P2X7R axis improves steatosis in alcoholic steatohepatitis Pubmed:35481896 |