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Mini Samples ELISA Kit for Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c)
- Product No.MEX132Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length3h
- Detection Range2.47-200ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.93ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 647
US$ 924
US$ 4158
US$ 7854
US$ 64680
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Specificity
This assay has high sensitivity and excellent specificity for detection of Mini Samples Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c).
No significant cross-reactivity or interference between Mini Samples Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Mini Samples Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 94-101 | 97 |
| EDTA plasma(n=5) | 97-105 | 101 |
| heparin plasma(n=5) | 83-95 | 92 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 82-96% | 91-99% | 79-92% | 91-102% |
| EDTA plasma(n=5) | 80-97% | 97-105% | 78-94% | 79-104% |
| heparin plasma(n=5) | 93-101% | 82-96% | 96-105% | 99-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×60µL | Assay Diluent A | 1×6mL |
| Detection Reagent B | 1×60µL | Assay Diluent B | 1×6mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×3mL |
| Wash Buffer (30 × concentrate) | 1×10mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well.
And then add 25μL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 50µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 50µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 25µL Stop Solution. Read at 450 nm immediately.
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| Magazine | Citations |
| Pediatric Diabetes | Circulating MOTS‐c levels are decreased in obese male children and adolescents and associated with insulin resistance Pubmed:29691953 |
| Rapid Commun Mass Spectrom | Development of a mass spectrometry‐based detection method for the mitochondrion‐derived peptide MOTS‐c in plasma samples for doping control purposes Pubmed: 30394592 |
| DIABETOLOGIA | Adiponectin treatment improves insulin resistance in mice by regulating the expression of the mitochondrial-derived peptide MOTS-c and its response to exercise via … Pubmed: 32880686 |
| Induction of hyperandrogenism and insulin resistance differentially modulates ferroptosis in uterine and placental tissues of pregnant rats | |
| FASEB JOURNAL | Neural cell©\derived plasma exosome protein abnormalities implicate mitochondrial impairment in first episodes of psychosis 33454965 |
| Biochim Biophys Acta Mol Basis Dis | MOTS-c interacts synergistically with exercise intervention to regulate PGC-1¦Á expression, attenuate insulin resistance and enhance glucose metabolism in mice via?¡ 33722744 |
| researchsquare | Evolution of Mitochondrial-Derived Peptides, Humanin and MOTSc and Changes in Insulin Sensitivity During Early Gestation |
| Mol Psychiatry | Abnormal levels of mitochondrial proteins in plasma neuronal extracellular vesicles in major depressive disorder 34471251 |
| Pharmacol Res | The Mitochondrial-Derived Peptide MOTS-c Relieves Hyperglycemia and Insulin Resistance in Gestational Diabetes Mellitus 34798268 |
| Ann Neurol | SARS‐CoV‐2 and mitochondrial proteins in neural‐derived exosomes of COVID‐19 Pubmed:35285072 |
| J Clin Med | Evolution of Mitochondrially Derived Peptides Humanin and MOTSc, and Changes in Insulin Sensitivity during Early Gestation in Women with and without Gestational … Pubmed:35683389 |