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Polyclonal Antibody to Collagen Type II (COL2)

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against COL2. It has been selected for its ability to recognize COL2 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
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Experimental Biology and Medicine Acidic pH conditions mimicking degenerative intervertebral discs impair the survival and biological behavior of human adipose-derived mesenchymal stem cells Rsmjournals: Source
PLoS ONE A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source
Stem Cell Research Therapy Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly Pubmed: 24739658
Spine (Phila Pa 1976). No effects of Hyperosmolar Culture medium on Tissue Regeneration by Human Degenerated Nucleus Pulposus Cells despite Upregulation Extracellular Matrix Genes. Pubmed:25856264
PLoS One. Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media Pubmed:Pmc4130592
Materials Science and Engineering: C Co-electrospun gelatin-poly (l-lactic acid) scaffolds: modulation of mechanical properties and chondrocyte response as a function of composition Pubmed:24433895
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Macromolecular Bioscience Highly Porous Gelatin Reinforced 3D Scaffolds for Articular Cartilage Regeneration PubMed: 25787871
Biol Res The role of MCP-1-CCR2 ligand-receptor axis in chondrocyte degradation and disease progress in knee osteoarthritis PubMed: 26578310
Cell Proliferation Effects of low oxygen tension on gene profile of soluble growth factors in co‐cultured adipose‐derived stromal cells and chondrocytes Pubmed:27090063
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Biomedical Materials An innovative co-axial system to electrospin in situ crosslinked gelatin nanofibers. Pubmed:26987305
Scientific Reports Chondrogenic Potential of Peripheral Blood Derived Mesenchymal Stem Cells Seeded on Demineralized Cancellous Bone Scaffolds pubmed:27821864
PLoS One. Self-complementary adeno-associated virus serotype 6 mediated knockdown of ADAMTS4 induces long-term and effective enhancement of aggrecan in degenerative human nucleus pulposus cells: A new therapeutic approach for intervertebral disc disorders. pubmed:28207788
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PLOS ONE Knockout of Apolipoprotein E in rabbit promotes premature intervertebral disc degeneration: A new in vivo model for therapeutic approaches of spinal disc disorders 10.1371:journal.pone.0187564
PLoS One Self-complementary adeno-associated virus serotype 6 mediated knockdown of ADAMTS4 induces long-term and effective enhancement of aggrecan in degenerative human nucleus pulposus cells: A new therapeutic approach for intervertebral disc disorders 10.1371:journal.pone.0172181
Platelets. Platelet-rich concentrate in serum-free medium enhances cartilage-specific extracellular matrix synthesis and reduces chondrocyte hypertrophy of human mesenchymal stromal cells encapsulated in alginate. pubmed:29090639
Molecular Medicine Reports Effects of GGCX overexpression on anterior cruciate ligament transection-induced osteoarthritis in rabbits Pubmed:29257344
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Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
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