Packages (Simulation)
Image (I)
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DAB staining on IHC-P; Sample: Rat Cerebrum Tissue Primary Ab: 10µg/ml Rabbit Anti-Rat OXA Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Image (II)
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Figure. Western Blot; Sample: Recombinant OXA, Rat.
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Polyclonal Antibody to Orexin A (OXA)
Hypocretin-1
- Product No.PAA607Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPA607Ra01-Recombinant Orexin A (OXA)
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration0.42mg/mL
- Organism Species Moren/a
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - DownloadInstruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 103
US$ 239
US$ 342
US$ 855
US$ 3420
For more details, please contact local distributors!
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against OXA. It has been selected for its ability to recognize OXA in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Magazine | Citations |
| Frontiers in Neurology | Neuro-Glial and Systemic Mechanisms of Pathological Responses in Rat Models of Primary Blast Overpressure Compared to “Composite” Blast PubMed: PMC3275793 |
| Neurological Sciences | Pentylenetetrazol-induced seizures are exacerbated by sleep deprivation through orexin receptor-mediated hippocampal cell proliferation Pubmed: 23852314 |
| Peptides | Orexin-A-induced ERK1/2 activation reverses impaired spatial learning and memory in pentylenetetrazol-kindled rats via OX1R-mediated hippocampal neurogenesis. Pubmed: 24321199 |
| Alcohol Alcohol | Orexin in Patients with Alcohol Dependence Treated for Relapse Prevention: A Pilot Study PubMed: 26597795 |
| Indian Journal of Medical Research and Pharmaceutical Sciences | Evaluation of Antidiabetic And Insulintropic Potential of Nigella Sativa Seeds Water Extract and /or Alpha Lipoic Acid in Type 2 Diabetic Rats Issues%20Pdf: Vol.2 |
| J Clin Res Pediatr Endocninol | Correlation of Brain Neuropeptide (Nesfatin-1 and Orexin-A) Concentrations with Anthropometric and Biochemical Parameters in Malnourished Children PubMed: 26831553 |
| Neural Regen Res | Resuscitation therapy for traumatic brain injury-induced coma in rats: mechanisms of median nerve electrical stimulation PubMed: 26170820 |
| Mol Med Rep | Wake-promoting actions of median nerve stimulation in TBI-induced coma: An investigation of orexin-A and orexin receptor 1 in the hypothalamic region PubMed: 26059340 |
| Mol Neurobiol | Application of the Co-culture Membrane System Pointed to a Protective Role of Catestatin on Hippocampal Plus Hypothalamic Neurons Exposed to Oxygen and Glucose Deprivation article:10.1007 |
| Neural Regeneration Research | Wake-promoting effects of vagus nerve stimulation after traumatic brain injury: upregulation of orexin-A and orexin receptor type 1 expression in the prefrontal … Pubmed:29557373 |
| Journal of Translational Medicine | Recurrent circadian fasting (RCF) improves blood pressure, biomarkers of cardiometabolic risk and regulates inflammation in men Pubmed: 31426866 |
| iraqi journal of science | Relationship Between Orexin-A and Insulin Resistance in Patients with Type 2 Diabetes Mellitus |
| Brain Sci | Changes in the Orexin System in Rats Exhibiting Learned Helplessness Behaviors 34942932 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA607Ra01 | Recombinant Orexin A (OXA) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA607Ra01 | Polyclonal Antibody to Orexin A (OXA) | WB; IHC; ICC; IP. |
| CEA607Ra | ELISA Kit for Orexin A (OXA) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA607Ra | Multiplex Assay Kit for Orexin A (OXA) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |