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CLIA Kit for Ciliary Neurotrophic Factor (CNTF)
HCNTF
- Product No.SCA021Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length2h, 40min
- Detection Range5.49-4,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 2.24pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 588
US$ 840
US$ 3780
US$ 7140
US$ 58800
For more details, please contact local distributors!
Specificity
This assay has high sensitivity and excellent specificity for detection of Ciliary Neurotrophic Factor (CNTF).
No significant cross-reactivity or interference between Ciliary Neurotrophic Factor (CNTF) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Ciliary Neurotrophic Factor (CNTF) and the recovery rates were calculated by comparing the measured value to the expected amount of Ciliary Neurotrophic Factor (CNTF) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 85-101 | 97 |
| EDTA plasma(n=5) | 78-98 | 88 |
| heparin plasma(n=5) | 80-89 | 86 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ciliary Neurotrophic Factor (CNTF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ciliary Neurotrophic Factor (CNTF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ciliary Neurotrophic Factor (CNTF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-95% | 97-104% | 82-101% | 82-98% |
| EDTA plasma(n=5) | 79-95% | 87-98% | 85-92% | 99-105% |
| heparin plasma(n=5) | 95-102% | 87-105% | 83-95% | 91-99% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| Substrate A | 1×10mL | Substrate B | 1×2mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.
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| Magazine | Citations |
| Stem?Cells.? | Ciliary Neurotrophic Factor Promotes the Activation of Corneal Epithelial Stem/Progenitor Cells and Accelerates Corneal Epithelial Wound Healing Pubmed:25546438 |
| Frontiers in Molecular Neuroscience | Rosiglitazone Exerts an Anti-depressive Effect in Unpredictable Chronic Mild-Stress-Induced Depressive Mice by Maintaining Essential Neuron Autophagy and Inhibiting Excessive Astrocytic Apoptosis. pubmed:28959186 |
| EXPERIMENTAL EYE RESEARCH | Mesenchymal stem cells provide paracrine neuroprotective resources that delay degeneration of co-cultured organotypic neuroretinal cultures Pubmed: 31108056 |