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CLIA Kit for Tissue Polypeptide Specific Antigen (TPS)

Specific M3 Epitope Of TPA

Specificity

This assay has high sensitivity and excellent specificity for detection of Tissue Polypeptide Specific Antigen (TPS).
No significant cross-reactivity or interference between Tissue Polypeptide Specific Antigen (TPS) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Tissue Polypeptide Specific Antigen (TPS) and the recovery rates were calculated by comparing the measured value to the expected amount of Tissue Polypeptide Specific Antigen (TPS) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 97-105 101
EDTA plasma(n=5) 83-95 91
heparin plasma(n=5) 95-103 98

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tissue Polypeptide Specific Antigen (TPS) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tissue Polypeptide Specific Antigen (TPS) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Tissue Polypeptide Specific Antigen (TPS) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-104% 80-94% 93-103% 91-105%
EDTA plasma(n=5) 82-99% 96-104% 78-101% 97-104%
heparin plasma(n=5) 78-92% 93-101% 89-103% 86-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

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Magazine Citations
Pancreatology Diagnostic Validity of Serum Macrophage Inhibitor Cytokine and Tissue Polypeptide-Specific Antigen in Pancreatobiliary Diseases Karger: 328963
Revista do Instituto de Medicina Tropical de São Paulo Vasoactive intestinal peptide degradation might influence Interleukin-17 expression in cardiac chagasic patients Pubmed: 30365640
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
SCB281Hu CLIA Kit for Tissue Polypeptide Specific Antigen (TPS) Chemiluminescent immunoassay for Antigen Detection.
LMB281Hu Multiplex Assay Kit for Tissue Polypeptide Specific Antigen (TPS) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.