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CLIA Kit for Alkaline Phosphatase (ALP)
AKP; ALKP; Basic Phosphatase
- Product No.SCB472Bo
- Organism SpeciesBos taurus; Bovine (Cattle) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length2h, 40min
- Detection Range68.6-50000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 25.3pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 706
US$ 1008
US$ 4536
US$ 8568
US$ 70560
For more details, please contact local distributors!
Specificity
This assay has high sensitivity and excellent specificity for detection of Alkaline Phosphatase (ALP).
No significant cross-reactivity or interference between Alkaline Phosphatase (ALP) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Alkaline Phosphatase (ALP) and the recovery rates were calculated by comparing the measured value to the expected amount of Alkaline Phosphatase (ALP) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 82-91 | 86 |
| EDTA plasma(n=5) | 80-96 | 82 |
| heparin plasma(n=5) | 83-90 | 87 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alkaline Phosphatase (ALP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alkaline Phosphatase (ALP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Alkaline Phosphatase (ALP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 94-103% | 85-96% | 82-91% | 78-101% |
| EDTA plasma(n=5) | 94-103% | 85-103% | 85-101% | 96-105% |
| heparin plasma(n=5) | 78-99% | 90-97% | 87-101% | 78-95% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| Substrate A | 1×10mL | Substrate B | 1×2mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.
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| Magazine | Citations |
| Clinical Oral Implants Research | Comparison of platelet rich fibrin and collagen as osteoblast-seeded scaffolds for bone tissue engineering applications Wiley: source |
| Die Pharmazie - An International Journal of Pharmaceutical Sciences | The role of atorvastatin in bone metabolism in male albino Wistar rats Ingenta: art00010 |
| 22 | Combined effect of strontium and zoledronate on hydroxyapatite structure and bone cell responses ScienceDirect: S0142961214003135 |
| Biomed Mater.? | Directing chondrogenic differentiation of mesenchymal stem cells with a solid-supported chitosan thermogel for cartilage tissue engineering Pubmed:24770944 |
| Journal of Colloid and Interface Science | Strontium and zoledronate hydroxyapatites graded composite coatings for bone prostheses Pubmed:25706198 |
| Bone | The active role of osteoporosis in the interaction between osteoblasts and bone metastases PubMed: 26057367 |
| Macromolecular Bioscience | Multi‐Layered Scaffolds for Osteochondral Tissue Engineering: In Vitro Response of Co‐Cultured Human Mesenchymal Stem Cells PubMed: 26126665 |
| journal of hard Tissue Biology | Biological Evaluation of a Prototype Material made of Polyglycolic Acid and Hydroxyapatite Article: Jhtb |
| Colloids Surf B Biointerfaces | Antiresorption implant coatings based on calcium alendronate and octacalcium phosphate deposited by matrix assisted pulsed laser evaporation PubMed: 26445021 |
| Gels | On the Mechanism of Drug Release from Polysaccharide Hydrogels Cross-Linked with Magnetite Nanoparticles by Applying Alternating Magnetic Fields: the Case of DOXO Delivery 2310-2861: 1 |
| Acta Biomater | Antioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: an in vitro osteoblast-osteoclast-endothelial cell co-culture study PubMed: 26689470 |
| Sci Rep | A composite scaffold of MSC affinity peptide-modified demineralized bone matrix particles and chitosan hydrogel for cartilage regeneration PubMed: 26632447 |
| Acta Biomaterialia | Antioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: An in vitro osteoblast–osteoclast–endothelial cell co-culture study Pubmed:26689470 |
| PLoS One. | Extracorporeal shock waves alone or combined with raloxifene promote bone formation and suppress resorption in ovariectomized rats. pubmed:28158228 |
| Scientific Reports | Adiponectin protects the rats liver against chronic intermittent hypoxia induced injury throughAMP-activated protein kinase pathway. pubmed:27678302 |
| J Cell Physiol. | An advanced tri-culture model to evaluate the dynamic interplay among osteoblasts, osteoclasts, and endothelial cells. pubmed:28240358 |
| Biomedicine & Pharmacotherapy | Anti-osteoporotic effects of an antidepressant tianeptine on ovariectomized rats S0753332216322752 |
| Journal of Inorganic Biochemistry | Biomimetic fabrication of antibacterial calcium phosphates mediated by polydopamine. pubmed:29049953 |
| Biomedicine & pharmacotherapy | Icaritin induces MC3T3-E1 subclone14 cell differentiation through estrogen receptor-mediated ERK1/2 and p38 signaling activation. pubmed:28742995 |
| Journal of Clinical Periodontology | Peroxisome proliferator‐activated receptor γ plays dual roles on experimental periodontitis in rats Pubmed:29574908 |
| Macromolecular Bioscience | Strontium‐Substituted Hydroxyapatite‐Gelatin Biomimetic Scaffolds Modulate Bone Cell Response Pubmed:29877029 |
| Journal of Inorganic Biochemistry | Gradient coatings of strontium hydroxyapatite/zinc β-tricalcium phosphate as a tool to modulate osteoblast/osteoclast response Pubmed:29525694 |
| DNA and Cell Biology | miRNA-133a-5p Inhibits the Expression of Osteoblast Differentiation-Associated Markers by Targeting the 3′ UTR of Pubmed:29359964 |
| Bone | The sole and combined effect of simvastatin and platelet rich fibrin as a filling material in induced bone defect in tibia of albino rats Pubmed: 30208342 |
| Experimental Gerontology | The decline of whole-body glucose metabolism in ovariectomized rats Pubmed: 30292771 |
| BMC Complementary and Alternative Medicine | Hepatoprotective activity of Erythrina× neillii leaf extract and characterization of its phytoconstituents Pubmed: 28095910 |
| Journal of Hematology & Oncology | Multiple myeloma-derived exosomes are enriched of amphiregulin (AREG) and activate the epidermal growth factor pathway in the bone microenvironment … Pubmed: 30621731 |
| JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS | CoCr porous scaffolds manufactured via selective laser melting in orthopedics: Topographical, mechanical, and biological characterization Pubmed: 30689288 |
| Chemico-Biological Interactions | Lead exposure inhibits osteoblastic differentiation and inactivates the canonical Wnt signal and recovery by icaritin in MC3T3-E1 subclone 14 cells Pubmed: 30731080 |
| Journal of Orthopaedic Surgery and Research | Macrophage inhibits the osteogenesis of fibroblasts in ultrahigh molecular weight polyethylene (UHMWPE) wear particle-induced osteolysis |
| Bangladesh Journal of Medical Science | Zn as the Factor of Alkaline Phosphatase in Periodontal Patients consuming Minangkabau food in West Sumatra, Indonesia |
| Molecular Therapy-Nucleic Acids | AAV-anti-miR-214 prevents collapse of the femoral head in osteonecrosis by regulating osteoblast and osteoclast activities Pubmed: 31739209 |
| Journal of Korean Neurosurgical Society | Rutin Improves Bone Histomorphometric Values by Reduction of Osteoclastic Activity in Osteoporosis Mouse Model Induced by Bilateral Ovariectomy Pubmed: 32172552 |
| Journal of Applied Biomaterials & Functional Materials | Effects of tricalcium silicate/sodium alginate/calcium sulfate hemihydrate composite cements on osteogenic performances in vitro and in vivo Pubmed: 32138579 |
| Nutrients | Osteoprotective Activity and Metabolite Fingerprint via UPLC/MS and GC/MS of Lepidium sativum in Ovariectomized Rats Pubmed: 32668691 |
| International Journal of Clinical and Experimental Pathology | Matrilin-2 is a novel prognostic marker in osteosarcoma |
| Revista Argentina de Clínica Psicológica | Research on the Mechanism of miR-142-5p's Promotion of the Function and Mineralization of Osteoblasts by Down-regulating the Expression of WWP1 Gene |
| МАРКЕРЫ КОСТНОГО РЕМОДЕЛИРОВАНИЯ ПРИ ЗАМЕЩЕНИИ ДЕФЕКТАТРАБЕКУЛЯРНОЙ КОСТНОЙ ТКАНИ РЕЗОРБИРУЕМЫМИ И … | |
| Ceramics International | Platinum nanoparticles supported on functionalized hydroxyapatite: Anti-oxidant properties and bone cells response |
| Bioelectromagnetics | Static Magnetic Field (2¨C4 T) Improves Bone Microstructure and Mechanical Properties by Coordinating Osteoblast/Osteoclast Differentiation in Mice 33655538 |
| PeerJ | From hair to liver: emerging application of hair follicle mesenchymal stem cell transplantation reverses liver cirrhosis by blocking the TGF-β/Smad signaling … Pubmed:35186473 |
| Cell Death Discov | ECM1 modified HF-MSCs targeting HSC attenuate liver cirrhosis by inhibiting the TGF-β/Smad signaling pathway Pubmed:35136027 |
| Int J Mol Sci | Antiosteoporotic Nanohydroxyapatite Zoledronate Scaffold Seeded with Bone Marrow Mesenchymal Stromal Cells for Bone Regeneration: A 3D In Vitro Model Pubmed:35682677 |
| Journal of Bionic Engineering | Effect of Antibacterial Enoxacin on the Properties of Injectable Nano-hydroxyapatite/Polyurethane Cement for Bone Repairing |
| Catalog No. | Related products for research use of Bos taurus; Bovine (Cattle) Organism species | Applications (RESEARCH USE ONLY!) |
| RPB472Bo01 | Recombinant Alkaline Phosphatase (ALP) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB472Bo01 | Polyclonal Antibody to Alkaline Phosphatase (ALP) | WB; IHC; ICC; IP. |
| LAB472Bo71 | Biotin-Linked Polyclonal Antibody to Alkaline Phosphatase (ALP) | WB; IHC; ICC. |
| SEB472Bo | ELISA Kit for Alkaline Phosphatase (ALP) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCB472Bo | CLIA Kit for Alkaline Phosphatase (ALP) | Chemiluminescent immunoassay for Antigen Detection. |
| LMB472Bo | Multiplex Assay Kit for Alkaline Phosphatase (ALP) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
| KSB472Bo01 | ELISA Kit DIY Materials for Alkaline Phosphatase (ALP) | Main materials for "Do It (ELISA Kit) Yourself". |