≡

CLIA Kit for Myosin Binding Protein C, Slow Type (MYBPC1)

MYBPCC; MYBPCS; C-protein, skeletal muscle slow isoform

Specificity

This assay has high sensitivity and excellent specificity for detection of Myosin Binding Protein C, Slow Type (MYBPC1).
No significant cross-reactivity or interference between Myosin Binding Protein C, Slow Type (MYBPC1) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myosin Binding Protein C, Slow Type (MYBPC1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myosin Binding Protein C, Slow Type (MYBPC1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

GIVEAWAYS

INCREMENT SERVICES

Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPD413Mu01 Recombinant Myosin Binding Protein C, Slow Type (MYBPC1) Positive Control; Immunogen; SDS-PAGE; WB.
RPD413Mu02 Recombinant Myosin Binding Protein C, Slow Type (MYBPC1) Positive Control; Immunogen; SDS-PAGE; WB.
PAD413Mu01 Polyclonal Antibody to Myosin Binding Protein C, Slow Type (MYBPC1) WB; IHC; ICC; IP.
PAD413Mu02 Polyclonal Antibody to Myosin Binding Protein C, Slow Type (MYBPC1) WB; IHC; ICC; IP.
SED413Mu ELISA Kit for Myosin Binding Protein C, Slow Type (MYBPC1) Enzyme-linked immunosorbent assay for Antigen Detection.
SCD413Mu CLIA Kit for Myosin Binding Protein C, Slow Type (MYBPC1) Chemiluminescent immunoassay for Antigen Detection.
LMD413Mu Multiplex Assay Kit for Myosin Binding Protein C, Slow Type (MYBPC1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.