≡

ELISA Kit for Carboxypeptidase B2 (CPB2)

TAFI; CPU; PCPB; Thrombin Activatable Fibrinolysis Inhibitor; Plasma Carboxypeptidase B; Carboxypeptidase U

Specificity

This assay has high sensitivity and excellent specificity for detection of Carboxypeptidase B2 (CPB2).
No significant cross-reactivity or interference between Carboxypeptidase B2 (CPB2) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Carboxypeptidase B2 (CPB2) and the recovery rates were calculated by comparing the measured value to the expected amount of Carboxypeptidase B2 (CPB2) in samples.

Matrix Recovery range (%) Average(%)
sodium citrate plasma(n=5) 99-105 102

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carboxypeptidase B2 (CPB2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carboxypeptidase B2 (CPB2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Carboxypeptidase B2 (CPB2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
sodium citrate plasma(n=5) 96-105% 90-98% 97-104% 94-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Taiwan J Obstet Gynecol.  Levels of thrombin-activatable fibrinolysis inhibitor and platelet-activating factor in recurrent pregnancy loss patients Pubmed:26927250
Experiental And Therapeutic Medicine Plasma proteins as potential targets of abnormal Savda syndrome in asthma patients treated with unique Uighur prescription pubmed:28672924
Clinics and Research in Hepatology and Gastroenterology Thrombin activatable fibrinolysis inhibitor as an indicator of the severity of acute pancreatitis Pubmed:22230218
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA615Hu01 Recombinant Carboxypeptidase B2 (CPB2) Positive Control; Immunogen; SDS-PAGE; WB.
APA615Hu01 Active Carboxypeptidase B2 (CPB2) Cell culture; Activity Assays.
PAA615Hu01 Polyclonal Antibody to Carboxypeptidase B2 (CPB2) WB; IHC; ICC; IP.
MAA615Hu22 Monoclonal Antibody to Carboxypeptidase B2 (CPB2) WB; IHC; ICC; IP.
SEA615Hu ELISA Kit for Carboxypeptidase B2 (CPB2) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA615Hu Multiplex Assay Kit for Carboxypeptidase B2 (CPB2) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.