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ELISA Kit for Kidney Injury Molecule 1 (Kim1)
HAVCR1; TIM1; TIMD1; HAVCR; Hepatitis A Virus Cellular Receptor 1; T Cell Immunoglobulin And Mucin Domain-Containing Protein 1; T-cell immunoglobulin mucin receptor 1
- Product No.SEA785Po
- Organism SpeciesSus scrofa; Porcine (Pig) Same name, Different species.
- Sample TypeTissue homogenates, cell lysates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range31.2-2,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 13.9pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 559
For more details, please contact local distributors! US$ 798 US$ 3591 US$ 6783 US$ 55860
Specificity
This assay has high sensitivity and excellent specificity for detection of Kidney Injury Molecule 1 (Kim1).
No significant cross-reactivity or interference between Kidney Injury Molecule 1 (Kim1) and analogues was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kidney Injury Molecule 1 (Kim1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kidney Injury Molecule 1 (Kim1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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Magazine | Citations |
Clinical Toxicology | Clinical implication of urinary neutrophil gelatinase-associated lipocalin and kidney injury molecule-1 in patients with acute paraquat intoxication PubMed: 19827907 |
Pediatric Nephrology | KIM-1 and NGAL: new markers of obstructive nephropathy SpringerLink: v461592787w5488x |
Pediatric Research | Urinary Neutrophil Gelatinase-Associated Lipocalin at Birth Predicts Early Renal Function in Very Low Birth Weight Infants LWW: 10000 |
Archives of Medical Science | Markers of kidney function in the elderly in relation to the new CKD-EPI formula for estimation of glomerular filtration rate AMS: source |
Nephrology | Targeting the AGE-RAGE axis improves renal function in the context of a healthy diet low in advanced glycation end-product content PubMed: 23046363 |
Nanotoxicology. | Comprehensive assessment of nephrotoxicity of intravenously administered sodium-oleate-coated ultra-small superparamagnetic iron oxide (USPIO) and titanium dioxide (TiO(2)) nanoparticles in rats. PubMed: 23272807 |
Toxicological Science | A Mouse Diversity Panel Approach Reveals the Potential for Clinical Kidney Injury Due to DB289 Not Predicted by Classical Rodent Models PubMed: PMC3498743 |
Disease Markers | Interleukin 18 as a Marker of Chronic Nephropathy in Children after Anticancer Treatment Hindawi: 369784 |
Journal of Biomarkers | Urinary Measurement of Neutrophil Gelatinase Associated Lipocalin and Kidney Injury Molecule-1 Helps Diagnose Acute Pyelonephritis in a Preclinical Model Hindawi: 413853 |
Advances in Life Science and Technology | Kidney injury molecule-1: a urinary biomarker for contrast induced acute kidney injury. Iiste: Source |
Nephrology | Urinary neutrophil gelatinase-associated lipocalin predicts graft loss after acute kidney injury in kidney transplant Pubmed: 24325180 |
Nephrology | Targeting the AGE-RAGE axis improves renal function in the context of a healthy diet low in advanced glycation end-product content. Pubmed: 23046363 |
BioMed Research International | The Renal Effects of Vanadate Exposure: Potential Biomarkers and Oxidative Stress as a Mechanism of Functional Renal Disorders—Preliminary Studies Hindawi: 740105 |
Life Sci. | Attenuation of renal ischemia/reperfusion injury by a?aí extract preconditioning in a rat model Pubmed:25476829 |
Diabetes & Vascular Disease Research | Urinary Kim-1 is a sensitive biomarker for the early stage of diabetic nephropathy in Otsuka Long-Evans Tokushima Fatty rats Sagepub:Source |
临床儿科杂志 | 中性粒细胞明胶酶相关脂质运载蛋白、肾损伤分子1和白介素18 在诊断心肺分流术后急性肾损伤中的意义 Jcp-Sh:Source |
Nephrology (Carlton). | Tubular urinary biomarkers do not identify aetiology of acute kidney injury in kidney transplant recipients Pubmed:24666430 |
PLoS One. | Cilostazol Renoprotective Effect: Modulation of PPAR-γ, NGAL, KIM-1 and IL-18 Underlies Its Novel Effect in a Model of Ischemia-Reperfusion Pubmed:24816434 |
BMC Nephrology | SYNbiotics Easing Renal failure by improving Gut microbiologY (SYNERGY): a protocol of placebo-controlled randomised cross-over trial Pubmed:24996842 |
Pediatric Nephrology | The diagnosis of febrile urinary tract infection in children may be facilitated by urinary biomarkers Pubmed:25127917 |
Inflammation.? | Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous Crisis Pubmed:25448261 |
Transworld Medical Journal | Kidney injury molecule-1: A urinary biomarker for contrast-induced acute kidney injury Tmjournals:Source |
Int J Clin Exp Pathol | Rosiglitazone attenuates renal injury caused by hyperlipidemic pancreatitis PubMed: 26191125 |
Int J Clin Exp Pathol | Inhalation of hydrogen gas ameliorates glyoxylate-induced calcium oxalate deposition and renal oxidative stress in mice PubMed: 26045773 |
Mol Med Rep | Vorinostat protects against calcium oxalate-induced kidney injury in mice PubMed: 26095064 |
Pediatr Nephrol | Role of new biomarkers for predicting renal scarring in vesicoureteral reflux: NGAL, KIM-1, and L-FABP PubMed: 26324091 |
Journal of Pediatric Urology | Urinary NGAL, KIM-1 and L-FABP concentrations in antenatal hydronephrosis PubMed: 26096437 |
Am J Physiol Renal Physiol | Intrarenal Renin-Angiotensin System Mediates Fatty Acid-Induced ER Stress in the Kidney PubMed: 26672616 |
Mol Med Rep. | Attenuation of renal ischemia/reperfusion injury by oleanolic acid preconditioning via its antioxidant, anti‑inflammatory, and anti‑apoptotic activities. Pubmed:27082705 |
Nephrol. Dial. Transplant. | Preconditioned suppression of prolyl-hydroxylases attenuates renal injury but increases mortality in septic murine models Pubmed:26908768 |
J Clin Lab Anal. | Kidney Injury Molecule Levels in Type 2 Diabetes Mellitus Pubmed:27132791 |
Med Sci Monit. | Protective Effect of Dihydromyricetin Against Lipopolysaccharide-Induced Acute Kidney Injury in a Rat Model pmc:PMC4754091 |
Diabetes. | Deficiency in Apoptosis-Inducing Factor Recapitulates Chronic Kidney Disease via Aberrant Mitochondrial Homeostasis Pubmed:26822084 |
Sci Rep. | Once daily administration of the SGLT2 inhibitor, empagliflozin, attenuates markers of renal fibrosis without improving albuminuria in diabetic db/db mice Pubmed:27226136 |
Journal of Applied Life Sciences International | Vitamins C and E Alleviate Nephrotoxicity-induced by Potassium Bromate in Rats Barakat812016JALSI28698.pdf |
Oxidative Medicine and Cellular Longevity | Synergic Effect of α-Mangostin on the Cytotoxicity of Cisplatin in a Cervical Cancer Model. pubmed:28053694 |
Molecular Medicine Reports | Attenuation of renal ischemia/reperfusion injury by oleanolic acid preconditioning via itsantioxidant, anti‑inflammatory, and anti‑apoptotic activities. pubmed:27082705 |
Journal of Agricultural and Food Chemistry | Protective Effects of DHA-PC against Vancomycin-Induced Nephrotoxicity through the Inhibition of Oxidative Stress and Apoptosis in BALB/c Mice pubmed:29254330 |
Clin Exp Pharmacol Physiol. | Early urinary biomarkers of renal tubular damage by a high-salt intake independent of blood pressure in normotensive rats. pubmed:29027259 |
Nephron - Physiology | Urinary Kidney Injury Molecule-1 but Not Urinary Neutrophil Gelatinase Associated Lipocalin Is Increased after Short Maximal Exercise 10.1159/000481179 |
Cellular Physiology and Biochemistry | Gum Acacia Improves Renal Function and Ameliorates Systemic Inflammation, Oxidative and Nitrosative Stress in Streptozotocin-Induced Diabetes in Rats … Pubmed:29550811 |
Frontiers in Pharmacology | Exploring the therapeutic mechanism of Desmodium styracifolium on oxalate crystal-induced kidney injuries using comprehensive approaches based on … Pubmed:29950996 |
Molecules | Effect of Pinocembrin Isolated from Mexican Brown Propolis on Diabetic Nephropathy Pubmed:29642511 |
American Journal of Physiology-Regulatory Integrative and Comparative Physiology | Modeling heart failure risk in diabetes and kidney disease: limitations and potential applications of transverse aortic constriction in high-fat-fed mice Pubmed:29443547 |
Biological Trace Element Research | Effect of Aqueous Extract of Glycyrrhiza glabra on the Biochemical Changes Induced by Cadmium Chloride in Rats Pubmed: 30251228 |
Cancer Research and Treatment | A Phage Display-Identified Peptide Selectively Binds to Kidney Injury Molecule-1(KIM-1) and Detects KIM-1-Overexpressing Tumors in vivo |
Archives of Medical Science | Urinary neutrophil gelatinase-associated lipocalin, kidney injury molecule-1, N-acetyl-β-D-glucosaminidase levels and mortality risk in septic patients with … Pubmed: 30393493 |
Biological & Pharmaceutical Bulletin | UPLC/MS-Based Metabolomics Investigation of the Protective Effect of Hydrogen Gas Inhalation on Mice with Calcium Oxalate-Induced Renal Injury Pubmed: 30381664 |
Kidney and Blood Pressure Research | The Kidney Specific Protein myo-Inositol Oxygenase, a Potential Biomarker for Diabetic Nephropathy Pubmed: 30504713 |
Clinica Chimica Acta | Urine markers of renal tubular injury in idiopathic membranous nephropathy: A cross sectional study Pubmed: 30684459 |
BMC Complementary and Alternative Medicine | Dual actions on gout flare and acute kidney injury along with enhanced renal transporter activities by Yokuininto, a Kampo medicine Pubmed: 30871515 |
2.583 | Application of ELISA Technique and Human Microsomes in the Search for 11β-Hydroxysteroid Dehydrogenase Inhibitors Doi: BioMed Research International |
Naunyn-Schmiedeberg's archives of pharmacology | Piceatannol protects against cisplatin nephrotoxicity via activation of Nrf2/HO-1 pathway and hindering NF-κB inflammatory cascade Pubmed: 31197431 |
Egyptian Journal of Zoology | Effect of Cucumis melo var. flexuosus leaves extract on renal oxidative injury and inflammation in diabetic male albino rats |
Environment International | Toxicity assessment due to prenatal and lactational exposure to lead, cadmium and mercury mixtures Pubmed: 31639605 |
Bioscience Reports | page Metabolomics study of fasudil on cisplatin-induced kidney injury Pubmed: 31670380 |
life sciences | MiR-214-3p exacerbates kidney damages and inflammation induced by hyperlipidemic pancreatitis complicated with acute renal injury Pubmed: 31790686 |
Arch Physiol Biochem | Protective effect of saxagliptin against renal ischaemia reperfusion injury in rats Pubmed: 31979992 |
clinical science | Delineating a role for the Mitochondrial Permeability Transition Pore in Diabetic Kidney Disease by targeting Cyclophilin D Pubmed: 31943002 |
LIFE SCIENCES | Rapamycin improves renal injury induced by Iodixanol in diabetic rats by deactivating the mTOR/p70S6K signaling pathway Pubmed: 32798557 |
INTERNATIONAL UROLOGY AND NEPHROLOGY | Serum NGAL, KIM-1, IL-18, L-FABP: new biomarkers in the diagnostics of acute kidney injury (AKI) following invasive cardiology procedures Pubmed: 32557377 |
Journal of Pediatric Urology | Can Serum Neutrophil Gelatinase Associated Lipocalin and Kidney Injury Molecule-1 Help in Decision Making for Surgery in Antenatally Dedected … Pubmed: 33139211 |
Ratlarda sodyum florid ile oluşturulan toksikasyonda Karvakol'ün böbrekler üzerine etkisi | |
Rationale and design of the intervention in PHOspholamban RElated CArdiomyopathy STudy (i-PHORECAST) | |
Pediatr Int | Early Kidney Injury in Immunoglobulin A Vasculitis: Role of Renal Biomarkers 33423326 |
J Int Med Res | Value of urinary kidney injury molecule-1 levels in predicting acute kidney injury in very low birth weight preterm infants 33372811 |
Biomed Res Int | Renal Protective Effect of Beluga Lentil Pretreatment for Ischemia-Reperfusion Injury 33604384 |
Nutrients | Targeting Methylglyoxal in Diabetic Kidney Disease Using the Mitochondria-Targeted Compound MitoGamide 33922959 |
J Am Soc Nephrol | Kim-1 Targeted Extracellular Vesicles: A New Therapeutic Platform for RNAi to Treat AKI 34127536 |
Heliyon | Anti-oxidant impact of Lisinopril and Enalapril against acute kidney injury induced by doxorubicin in male Wistar rats: involvement of kidney injury molecule-1 33506137 |
Journal of Pharmacological Sciences | Pentoxifylline treatment alleviates kidney ischemia/reperfusion injury: Novel involvement of galectin-3 and ASK-1/JNK & ERK1/2/NF-¦ÊB/HMGB-1 trajectories 34030796 |
Int Urol Nephrol | Could urinary kidney injury molecule-1 be a good marker in subclinical acute kidney injury in mild to moderate COVID-19 infection? 34213713 |
Intensive Care Medicine Experimental | Preservation of renal endothelial integrity and reduction of renal edema by aprotinin does not preserve renal perfusion and function following experimental … 34169407 |
iscience | Kidney disease risk factors do not explain impacts of low dietary protein on kidney function and structure 34820603 |
Catalog No. | Related products for research use of Sus scrofa; Porcine (Pig) Organism species | Applications (RESEARCH USE ONLY!) |
RPA785Po01 | Recombinant Kidney Injury Molecule 1 (Kim1) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA785Po01 | Polyclonal Antibody to Kidney Injury Molecule 1 (Kim1) | WB; IHC; ICC; IP. |
SEA785Po | ELISA Kit for Kidney Injury Molecule 1 (Kim1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA785Po | Multiplex Assay Kit for Kidney Injury Molecule 1 (Kim1) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |