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ELISA Kit for Arachidonate-15-Lipoxygenase (ALOX15)
LOX15; 15-LOX2; 15-LOX1; 15-LOX; 12-LOX; ALOX15; 15-LO; 15-LPG; LOG15; Lipoxygenase 15; Arachidonate 12-lipoxygenase, leukocyte-type; Arachidonate omega-6 lipoxygenase
- Product No.SEA891Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample Typetissue homogenates, cell lysates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range1.56-100ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.55ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 441
US$ 630
US$ 2835
US$ 5355
US$ 44100
For more details, please contact local distributors!
Specificity
This assay has high sensitivity and excellent specificity for detection of Arachidonate-15-Lipoxygenase (ALOX15).
No significant cross-reactivity or interference between Arachidonate-15-Lipoxygenase (ALOX15) and analogues was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Arachidonate-15-Lipoxygenase (ALOX15) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Arachidonate-15-Lipoxygenase (ALOX15) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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| Magazine | Citations |
| Physiology & Biochemistry | Oxidized Low-Density Lipoprotein and Cell Adhesion Molecules Following Exercise Training pubmed:29190851 |
| Measurement of the expression of the ALOX15 gene in wounds undergoing negative pressure wound therapy |