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ELISA Kit for Sialoadhesin (SN)

CD169; SIGLEC1; Sialic Acid Binding Ig Like Lectin 1

Specificity

This assay has high sensitivity and excellent specificity for detection of Sialoadhesin (SN).
No significant cross-reactivity or interference between Sialoadhesin (SN) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Sialoadhesin (SN) and the recovery rates were calculated by comparing the measured value to the expected amount of Sialoadhesin (SN) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 82-105 85
EDTA plasma(n=5) 83-92 87
heparin plasma(n=5) 88-96 91

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sialoadhesin (SN) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sialoadhesin (SN) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Sialoadhesin (SN) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 78-89% 98-105% 98-105% 91-101%
EDTA plasma(n=5) 79-102% 81-102% 78-92% 98-105%
heparin plasma(n=5) 95-102% 83-101% 85-101% 83-91%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
World Allergy Organization Journal Diagnosis and management of nonallergic rhinitis with eosinophilia syndrome using cystatin SN together with symptoms Pubmed: 32577150
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY Prognostic and pharmacologic value of cystatin SN for chronic rhinosinusitis with nasal polyps 33675819
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
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