≡

ELISA Kit for Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1)

CHR-NA1; N-AChRA1; NAChRA1; ACHRA; ACHRD; CMS2A; FCCMS; SCCMS; Acetylcholine receptor subunit alpha; N-AChR-A1; Neuronal Acetylcholine Receptor Alpha 1,Muscle

Specificity

This assay has high sensitivity and excellent specificity for detection of Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1).
No significant cross-reactivity or interference between Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
International Journal of Peptides Effects of Endurance and Resistance Training on Calcitonin Gene-Related Peptide and Acetylcholine Receptor at Slow and Fast Twitch Skeletal Muscles and Sciatic Nerve in Male Wistar Rats PubMed: PMC3382945
Progress in Neuro-Psychopharmacology & Biological Psychiatry Effects of vortioxetine on biomarkers associated with glutamatergic activity in an SSRI insensitive model of depression in female rats pubmed:29269186
Saudi Pharmaceutical Journal Sukkari dates seed improves type-2 diabetes mellitus-induced memory impairment by reducing blood glucose levels and enhancing brain cholinergic … Pubmed:35812141
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA981Hu01 Recombinant Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1) Positive Control; Immunogen; SDS-PAGE; WB.
PAA981Hu01 Polyclonal Antibody to Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1) WB; IHC; ICC; IP.
MAA981Hu21 Monoclonal Antibody to Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1) WB; IHC; ICC; IP.
SEA981Hu ELISA Kit for Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA981Hu CLIA Kit for Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1) Chemiluminescent immunoassay for Antigen Detection.
LMA981Hu Multiplex Assay Kit for Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.