≡

ELISA Kit for Tubulin Polymerization Promoting Protein (TPPP)

TPPP1; p25alpha; p25; TPPP/p25; Brain Specific Protein p25 Alpha; 25 kDa brain-specific protein

Specificity

This assay has high sensitivity and excellent specificity for detection of Tubulin Polymerization Promoting Protein (TPPP).
No significant cross-reactivity or interference between Tubulin Polymerization Promoting Protein (TPPP) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tubulin Polymerization Promoting Protein (TPPP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tubulin Polymerization Promoting Protein (TPPP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA993Mu02 Recombinant Tubulin Polymerization Promoting Protein (TPPP) Positive Control; Immunogen; SDS-PAGE; WB.
RPA993Mu01 Recombinant Tubulin Polymerization Promoting Protein (TPPP) Positive Control; Immunogen; SDS-PAGE; WB.
EPA993Mu61 Eukaryotic Tubulin Polymerization Promoting Protein (TPPP) Positive Control; Immunogen; SDS-PAGE; WB.
APA993Mu61 Active Tubulin Polymerization Promoting Protein (TPPP) Cell culture; Activity Assays.
PAA993Mu02 Polyclonal Antibody to Tubulin Polymerization Promoting Protein (TPPP) WB; IHC; ICC; IP.
PAA993Mu01 Polyclonal Antibody to Tubulin Polymerization Promoting Protein (TPPP) WB; IHC; ICC; IP.
SEA993Mu ELISA Kit for Tubulin Polymerization Promoting Protein (TPPP) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA993Mu Multiplex Assay Kit for Tubulin Polymerization Promoting Protein (TPPP) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.