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ELISA Kit for Glypican 1 (GPC1)

Glypican Proteoglycan 1; Secreted glypican-1

Specificity

This assay has high sensitivity and excellent specificity for detection of Glypican 1 (GPC1).
No significant cross-reactivity or interference between Glypican 1 (GPC1) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Glypican 1 (GPC1) and the recovery rates were calculated by comparing the measured value to the expected amount of Glypican 1 (GPC1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 80-99 88
EDTA plasma(n=5) 97-105 101
heparin plasma(n=5) 80-104 98

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glypican 1 (GPC1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glypican 1 (GPC1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Glypican 1 (GPC1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 89-98% 78-95% 89-97% 87-97%
EDTA plasma(n=5) 94-101% 82-98% 92-105% 91-98%
heparin plasma(n=5) 96-105% 84-99% 91-103% 85-95%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
Intensive Care Medicine Experimental Elevated plasma glypicans are associated with organ failure in patients with infection Pubmed: 30618011
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPB032Mu01 Recombinant Glypican 1 (GPC1) Positive Control; Immunogen; SDS-PAGE; WB.
RPB032Mu02 Recombinant Glypican 1 (GPC1) Positive Control; Immunogen; SDS-PAGE; WB.
APB032Mu01 Active Glypican 1 (GPC1) Cell culture; Activity Assays.
APB032Mu02 Active Glypican 1 (GPC1) Cell culture; Activity Assays.
PAB032Mu02 Polyclonal Antibody to Glypican 1 (GPC1) WB; IHC; ICC; IP.
PAB032Mu01 Polyclonal Antibody to Glypican 1 (GPC1) WB; IHC; ICC; IP.
LAB032Mu71 Biotin-Linked Polyclonal Antibody to Glypican 1 (GPC1) WB; IHC; ICC.
SEB032Mu ELISA Kit for Glypican 1 (GPC1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB032Mu Multiplex Assay Kit for Glypican 1 (GPC1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.