≡

ELISA Kit for Antidiuretic Hormone (ADH)

ARVP; AVP; VP; Arginine Vasopressin; Arg-Vasopressin; Antidiuretic Hormone; Diabetes Insipidus; Neurohypophyseal; Argipressin

Specificity

This assay has high sensitivity and excellent specificity for detection of Antidiuretic Hormone (ADH).
No significant cross-reactivity or interference between Antidiuretic Hormone (ADH) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Antidiuretic Hormone (ADH) and the recovery rates were calculated by comparing the measured value to the expected amount of Antidiuretic Hormone (ADH) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 86-95 90
EDTA plasma(n=5) 99-105 102
heparin plasma(n=5) 80-90 86

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Antidiuretic Hormone (ADH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Antidiuretic Hormone (ADH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Antidiuretic Hormone (ADH) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 82-93% 98-105% 97-105% 81-104%
EDTA plasma(n=5) 78-96% 87-101% 80-96% 91-105%
heparin plasma(n=5) 84-104% 94-102% 83-103% 92-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Neuroscience Angiotensin II Type 1a Receptors in the Subfornical Organ Modulate Neuroinflammation in the Hypothalamic Paraventricular Nucleus in Heart Failure Rats Pubmed:29684507
American Journal of Physiology-Renal Physiology Chronic nicotine exposure reduces klotho expression and triggers different renal and hemodynamic responses in -haploinsufficient mice Pubmed:29363324
The Eurasian Journal of Medicine Effects of Conivaptan versus Mannitol on Post-Ischemic Brain Injury and Edema
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
CPB139Hu21 OVA Conjugated Antidiuretic Hormone (ADH) Immunogen; SDS-PAGE; WB.
PAB139Hu01 Polyclonal Antibody to Antidiuretic Hormone (ADH) WB; IHC; ICC; IP.
LAB139Hu71 Biotin-Linked Polyclonal Antibody to Antidiuretic Hormone (ADH) WB; IHC; ICC.
SEB139Hu ELISA Kit for Antidiuretic Hormone (ADH) Enzyme-linked immunosorbent assay for Antigen Detection.
CEB139Hu ELISA Kit for Antidiuretic Hormone (ADH) Enzyme-linked immunosorbent assay for Antigen Detection.
HEB139Hu High Sensitive ELISA Kit for Antidiuretic Hormone (ADH) Enzyme-linked immunosorbent assay for Antigen Detection.
CCB139Hu CLIA Kit for Antidiuretic Hormone (ADH) Chemiluminescent immunoassay for Antigen Detection.
LMB139Hu Multiplex Assay Kit for Antidiuretic Hormone (ADH) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSB139Hu11 ELISA Kit DIY Materials for Antidiuretic Hormone (ADH) Main materials for "Do It (ELISA Kit) Yourself".