≡

ELISA Kit for Membrane Spanning 4 Domains Subfamily A, Member 1 (CD20)

MS4A1; MS4-A1; B1; Bp35; LEU16; MS4-A2; S7; B-lymphocyte antigen CD20; B-lymphocyte surface antigen B1

Specificity

This assay has high sensitivity and excellent specificity for detection of Membrane Spanning 4 Domains Subfamily A, Member 1 (CD20).
No significant cross-reactivity or interference between Membrane Spanning 4 Domains Subfamily A, Member 1 (CD20) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Membrane Spanning 4 Domains Subfamily A, Member 1 (CD20) and the recovery rates were calculated by comparing the measured value to the expected amount of Membrane Spanning 4 Domains Subfamily A, Member 1 (CD20) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 79-102 85
EDTA plasma(n=5) 90-98 95
heparin plasma(n=5) 78-104 82

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Membrane Spanning 4 Domains Subfamily A, Member 1 (CD20) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Membrane Spanning 4 Domains Subfamily A, Member 1 (CD20) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Membrane Spanning 4 Domains Subfamily A, Member 1 (CD20) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 90-105% 97-104% 95-105% 89-101%
EDTA plasma(n=5) 95-104% 90-104% 80-101% 90-104%
heparin plasma(n=5) 85-104% 93-105% 87-96% 78-95%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPB148Hu01 Recombinant Membrane Spanning 4 Domains Subfamily A, Member 1 (CD20) Positive Control; Immunogen; SDS-PAGE; WB.
EPB148Hu61 Eukaryotic Membrane Spanning 4 Domains Subfamily A, Member 1 (CD20) Positive Control; Immunogen; SDS-PAGE; WB.
PAB148Hu01 Polyclonal Antibody to Membrane Spanning 4 Domains Subfamily A, Member 1 (CD20) WB; IHC; ICC; IP.
RAB148Hu21 Recombinant Antibody to Membrane Spanning 4 Domains Subfamily A, Member 1 (CD20) WB; IF; ICC; IHC; IP; FCM.
MAB148Hu21 Monoclonal Antibody to Membrane Spanning 4 Domains Subfamily A, Member 1 (CD20) WB; IHC; ICC; IP.
FAB148Hu02 Anti-Membrane Spanning 4 Domains Subfamily A, Member 1 (CD20) Monoclonal Antibody FCM
FAB148Hu04 Anti-Membrane Spanning 4 Domains Subfamily A, Member 1 (CD20) Monoclonal Antibody FCM
FAB148Hu06 Anti-Membrane Spanning 4 Domains Subfamily A, Member 1 (CD20) Monoclonal Antibody FCM
FAB148Hu08 Anti-Membrane Spanning 4 Domains Subfamily A, Member 1 (CD20) Monoclonal Antibody FCM
FAB148Hu82 FITC-Linked Anti-Membrane Spanning 4 Domains Subfamily A, Member 1 (CD20) Monoclonal Antibody FCM
SEB148Hu ELISA Kit for Membrane Spanning 4 Domains Subfamily A, Member 1 (CD20) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB148Hu Multiplex Assay Kit for Membrane Spanning 4 Domains Subfamily A, Member 1 (CD20) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.