≡

ELISA Kit for Secretory Leukocyte Peptidase Inhibitor (SLPI)

ALK1; ALP; MPI; BLPI; HUSI; HUSI-I; WAP4; WFDC4; Antileukoproteinase; Seminal proteinase inhibitor; Mucus proteinase inhibitor; WAP four-disulfide core domain protein 4

Specificity

This assay has high sensitivity and excellent specificity for detection of Secretory Leukocyte Peptidase Inhibitor (SLPI).
No significant cross-reactivity or interference between Secretory Leukocyte Peptidase Inhibitor (SLPI) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Secretory Leukocyte Peptidase Inhibitor (SLPI) and the recovery rates were calculated by comparing the measured value to the expected amount of Secretory Leukocyte Peptidase Inhibitor (SLPI) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 97-104 101
EDTA plasma(n=5) 80-103 101
heparin plasma(n=5) 85-102 98

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Secretory Leukocyte Peptidase Inhibitor (SLPI) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Secretory Leukocyte Peptidase Inhibitor (SLPI) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Secretory Leukocyte Peptidase Inhibitor (SLPI) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-99% 89-98% 79-98% 80-104%
EDTA plasma(n=5) 80-93% 84-95% 92-99% 94-103%
heparin plasma(n=5) 78-98% 80-93% 81-104% 78-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Human Immunology Serum alarm antiproteases in systemic sclerosis patients pubmed:28606626
The Journal of Toxicological Sciences Editor's Highlight: Candidate Risk Factors and Mechanisms for Tolvaptan-Induced Liver Injury Are Identified Using a Collaborative Cross Approach pubmed:28115652
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPB312Ra01 Recombinant Secretory Leukocyte Peptidase Inhibitor (SLPI) Positive Control; Immunogen; SDS-PAGE; WB.
PAB312Ra01 Polyclonal Antibody to Secretory Leukocyte Peptidase Inhibitor (SLPI) WB; IHC; ICC; IP.
SEB312Ra ELISA Kit for Secretory Leukocyte Peptidase Inhibitor (SLPI) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB312Ra Multiplex Assay Kit for Secretory Leukocyte Peptidase Inhibitor (SLPI) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.