≡

ELISA Kit for Growth Factor Receptor Bound Protein 2 (Grb2)

ASH; NCKAP2; EGFRBP-GRB2; Grb3-3; Adapter protein GRB2; Protein Ash; SH2/SH3 adapter GRB2

Specificity

This assay has high sensitivity and excellent specificity for detection of Growth Factor Receptor Bound Protein 2 (Grb2).
No significant cross-reactivity or interference between Growth Factor Receptor Bound Protein 2 (Grb2) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Factor Receptor Bound Protein 2 (Grb2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Factor Receptor Bound Protein 2 (Grb2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Eur Neuropsychopharmacol. Regulation of insulin receptor phosphorylation in the brains of prenatally stressed rats: New insight into the benefits of antidepressant drug treatment. pubmed:28063625
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPC514Hu01 Recombinant Growth Factor Receptor Bound Protein 2 (Grb2) Positive Control; Immunogen; SDS-PAGE; WB.
EPC514Hu61 Eukaryotic Growth Factor Receptor Bound Protein 2 (Grb2) Positive Control; Immunogen; SDS-PAGE; WB.
APC514Hu61 Active Growth Factor Receptor Bound Protein 2 (Grb2) Cell culture; Activity Assays.
PAC514Hu06 Polyclonal Antibody to Growth Factor Receptor Bound Protein 2 (Grb2) WB; IHC; ICC; IP.
PAC514Hu01 Polyclonal Antibody to Growth Factor Receptor Bound Protein 2 (Grb2) WB; IHC; ICC; IP.
MAC514Hu22 Monoclonal Antibody to Growth Factor Receptor Bound Protein 2 (Grb2) WB; IHC; ICC; IP.
SEC514Hu ELISA Kit for Growth Factor Receptor Bound Protein 2 (Grb2) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC514Hu Multiplex Assay Kit for Growth Factor Receptor Bound Protein 2 (Grb2) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.