≡

ELISA Kit for Surfactant Associated Protein G (SPG)

SP-G; SFTPG; SFTA2; Surfactant Associated 2

Specificity

This assay has high sensitivity and excellent specificity for detection of Surfactant Associated Protein G (SPG).
No significant cross-reactivity or interference between Surfactant Associated Protein G (SPG) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Surfactant Associated Protein G (SPG) and the recovery rates were calculated by comparing the measured value to the expected amount of Surfactant Associated Protein G (SPG) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 78-93 83
EDTA plasma(n=5) 90-104 93
heparin plasma(n=5) 96-105 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Surfactant Associated Protein G (SPG) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Surfactant Associated Protein G (SPG) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Surfactant Associated Protein G (SPG) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 93-103% 99-105% 78-92% 93-101%
EDTA plasma(n=5) 80-92% 83-102% 85-99% 80-98%
heparin plasma(n=5) 96-104% 86-101% 86-102% 78-93%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Molecular Neurobiology Localization, Occurrence, and CSF Changes of SP-G, a New Surface Active Protein with Assumable Immunoregulatory Functions in the CNS Pubmed:30032421
Biomolecules Surfactant Protein-G in Wildtype and 3xTg-AD Mice: Localization in the Forebrain, Age-Dependent Hippocampal Dot-like Deposits and Brain Content Pubmed:35053244
Int J Mol Sci Regionally Altered Immunosignals of Surfactant Protein-G, Vascular and Non-Vascular Elements of the Neurovascular Unit after Experimental Focal Cerebral … Pubmed:35682557
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPD755Hu01 Recombinant Surfactant Associated Protein G (SPG) Positive Control; Immunogen; SDS-PAGE; WB.
PAD755Hu01 Polyclonal Antibody to Surfactant Associated Protein G (SPG) WB
SED755Hu ELISA Kit for Surfactant Associated Protein G (SPG) Enzyme-linked immunosorbent assay for Antigen Detection.
LMD755Hu Multiplex Assay Kit for Surfactant Associated Protein G (SPG) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.