≡

ELISA Kit for Defensin Alpha 1B (DEFa1B)

HNP-1; DEFA2; MRS; Neutrophil defensin 1

Specificity

This assay has high sensitivity and excellent specificity for detection of Defensin Alpha 1B (DEFa1B).
No significant cross-reactivity or interference between Defensin Alpha 1B (DEFa1B) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Defensin Alpha 1B (DEFa1B) and the recovery rates were calculated by comparing the measured value to the expected amount of Defensin Alpha 1B (DEFa1B) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 96-105 101
EDTA plasma(n=5) 85-98 94
heparin plasma(n=5) 89-102 98

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Defensin Alpha 1B (DEFa1B) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Defensin Alpha 1B (DEFa1B) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Defensin Alpha 1B (DEFa1B) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 90-104% 94-105% 89-97% 94-102%
EDTA plasma(n=5) 80-90% 93-101% 80-89% 82-91%
heparin plasma(n=5) 81-104% 88-104% 87-101% 88-96%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Canadian Respiratory Journal Human Neutrophil Defensin-1,-3, and-4 Are Elevated in Nasal Aspirates from Children with Naturally Occurring Adenovirus Infection 10.1155:2018/1038593
LIFE SCIENCES DEFA1B inhibits ZIKV replication and retards cell cycle progression through interaction with ORC1 Pubmed: 33075374
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPE136Hu01 Recombinant Defensin Alpha 1B (DEFa1B) Positive Control; Immunogen; SDS-PAGE; WB.
PAE136Hu01 Polyclonal Antibody to Defensin Alpha 1B (DEFa1B) WB; ICC; IHC-P; IHC-F; ELISA.
MAE136Hu21 Monoclonal Antibody to Defensin Alpha 1B (DEFa1B) WB; IHC; ICC; IP.
SEE136Hu ELISA Kit for Defensin Alpha 1B (DEFa1B) Enzyme-linked immunosorbent assay for Antigen Detection.
LME136Hu Multiplex Assay Kit for Defensin Alpha 1B (DEFa1B) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.