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ELISA Kit for Folate Receptor 1, Adult (FOLR1)

FBP; FOLR; FR-alpha; MOv18; Adult folate-binding protein; KB cells FBP; Ovarian tumor-associated antigen MOv18

Specificity

This assay has high sensitivity and excellent specificity for detection of Folate Receptor 1, Adult (FOLR1).
No significant cross-reactivity or interference between Folate Receptor 1, Adult (FOLR1) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Folate Receptor 1, Adult (FOLR1) and the recovery rates were calculated by comparing the measured value to the expected amount of Folate Receptor 1, Adult (FOLR1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 78-102 82
EDTA plasma(n=5) 89-104 95
heparin plasma(n=5) 92-101 95

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Folate Receptor 1, Adult (FOLR1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Folate Receptor 1, Adult (FOLR1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Folate Receptor 1, Adult (FOLR1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 78-97% 78-96% 93-105% 98-105%
EDTA plasma(n=5) 95-102% 88-97% 78-99% 79-93%
heparin plasma(n=5) 96-104% 79-95% 89-97% 96-104%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
Journal of maternal-fetal & neonatal medicine Association of folic acid receptor α in maternal serum with neural tube defects Pubmed: 24094304
Tumour Biol. Quantitation of rare circulating tumor cells by folate receptor α ligand-targeted PCR in bladder transitional cell carcinoma and its potential diagnostic significance. Pubmed: 24771263
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
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