≡

ELISA Kit for Microphthalmia Associated Transcription Factor (MITF)

WS2A; MI; bHLHe32; Homolog Of Mouse Microphthalmia; Class E basic helix-loop-helix protein 32

Specificity

This assay has high sensitivity and excellent specificity for detection of Microphthalmia Associated Transcription Factor (MITF).
No significant cross-reactivity or interference between Microphthalmia Associated Transcription Factor (MITF) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Microphthalmia Associated Transcription Factor (MITF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Microphthalmia Associated Transcription Factor (MITF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Toxicology in Vitro Melanogenesis and antioxidant defense system in normal human melanocytes cultured in the presence of chlorpromazine Pubmed:25449126
Naunyn Schmiedebergs Arch Pharmacol Effect of thioridazine on antioxidant status of HEMn-DP melanocytes PubMed: 26105001
Acta Poloniae Pharmaceutica ñ Drug Research FLUPHENAZINE AND PERPHENAZINE IMPACT ON MELANOGENESIS AND ANTIOXIDANT ENZYMES ACTIVITY IN NORMAL HUMAN MELANOCYTES 903.pdf
Die Pharmazie-An International Journal of Pharmaceutical Sciences Prochlorperazine interaction with melanin and melanocytes art00007
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPH645Hu01 Recombinant Microphthalmia Associated Transcription Factor (MITF) Positive Control; Immunogen; SDS-PAGE; WB.
PAH645Hu01 Polyclonal Antibody to Microphthalmia Associated Transcription Factor (MITF) WB; IHC; ICC; IP.
MAH645Hu21 Monoclonal Antibody to Microphthalmia Associated Transcription Factor (MITF) WB; IHC; ICC; IP.
SEH645Hu ELISA Kit for Microphthalmia Associated Transcription Factor (MITF) Enzyme-linked immunosorbent assay for Antigen Detection.
LMH645Hu Multiplex Assay Kit for Microphthalmia Associated Transcription Factor (MITF) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.