≡

ELISA Kit for Succinate Dehydrogenase Complex Subunit A (SDHA)

SDH2; SDHF; FP; Flavoprotein; Flavoprotein subunit of complex II; Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial

Specificity

This assay has high sensitivity and excellent specificity for detection of Succinate Dehydrogenase Complex Subunit A (SDHA).
No significant cross-reactivity or interference between Succinate Dehydrogenase Complex Subunit A (SDHA) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Succinate Dehydrogenase Complex Subunit A (SDHA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Succinate Dehydrogenase Complex Subunit A (SDHA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Cell Stem Cell Macrophage-derived extracellular succinate licenses neural stem cells to suppress chronic neuroinflammation Pubmed:29478844
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPJ784Mu01 Recombinant Succinate Dehydrogenase Complex Subunit A (SDHA) Positive Control; Immunogen; SDS-PAGE; WB.
RPJ784Mu02 Recombinant Succinate Dehydrogenase Complex Subunit A (SDHA) Positive Control; Immunogen; SDS-PAGE; WB.
PAJ784Mu01 Polyclonal Antibody to Succinate Dehydrogenase Complex Subunit A (SDHA) WB; IHC; IF
PAJ784Mu02 Polyclonal Antibody to Succinate Dehydrogenase Complex Subunit A (SDHA) WB; IHC; ICC; IP.
MAJ784Mu21 Monoclonal Antibody to Succinate Dehydrogenase Complex Subunit A (SDHA) WB; IF
MAJ784Mu22 Monoclonal Antibody to Succinate Dehydrogenase Complex Subunit A (SDHA) WB; IF
MAJ784Mu23 Monoclonal Antibody to Succinate Dehydrogenase Complex Subunit A (SDHA) WB
SEJ784Mu ELISA Kit for Succinate Dehydrogenase Complex Subunit A (SDHA) Enzyme-linked immunosorbent assay for Antigen Detection.
LMJ784Mu Multiplex Assay Kit for Succinate Dehydrogenase Complex Subunit A (SDHA) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.