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ELISA Kit for Calprotectin (CALPRO)
MRP-8/MRP-14; S100A8/A9
- Product No.SEK504Ca
- Organism SpeciesCanis familiaris; Canine (Dog) Same name, Different species.
- Sample Typeserum, plasma and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.78-50ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.29ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 560
US$ 800
US$ 3600
US$ 6800
US$ 56000
For more details, please contact local distributors!
Specificity
This assay has high sensitivity and excellent specificity for detection of Calprotectin (CALPRO).
No significant cross-reactivity or interference between Calprotectin (CALPRO) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Calprotectin (CALPRO) and the recovery rates were calculated by comparing the measured value to the expected amount of Calprotectin (CALPRO) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 94-102 | 97 |
| EDTA plasma(n=5) | 88-104 | 101 |
| heparin plasma(n=5) | 86-102 | 96 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calprotectin (CALPRO) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calprotectin (CALPRO) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Calprotectin (CALPRO) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 96-104% | 93-101% | 89-103% | 86-105% |
| EDTA plasma(n=5) | 95-102% | 85-92% | 86-98% | 90-98% |
| heparin plasma(n=5) | 79-89% | 92-99% | 85-104% | 94-103% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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| Magazine | Citations |
| Medicine (Baltimore) | A Novel Combination of Calprotectin and CXCL12 for Predicting Malignancy in Patients with Exudative Pleural Effusion PubMed: 26632726 |
| Neurochemical Research | Effects of (-)-Sesamin on Chronic Stress-Induced Anxiety Disorders in Mice pubmed:27995494 |
| International journal of molecular sciences | Immune Modulating Topical S100A8/A9 Inhibits Growth of Pseudomonas aeruginosa and Mitigates Biofilm Infection in Chronic Wounds. pubmed:28672877 |
| Analytical Chemistry | Multiplex Immuno-MALDI-TOF MS for Targeted Quantification of Protein Biomarkers and Their Proteoforms Related to Inflammation and Renal Dysfunction Pubmed:29420882 |
| JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH | Maternal serum calprotectin level in intrahepatic cholestasis of pregnancy 34254410 |
| Bull Exp Biol Med | Influence of Rectal Ozone Application on the Intensity of Free Radical Destruction of Lipids and Intestinal Proteins in the Dynamics of Experimental Colitis Pubmed:35622259 |
| Serum Calprotectin and Iron Metabolism Biomarker Levels in Behçet's Disease |
| Catalog No. | Related products for research use of Canis familiaris; Canine (Dog) Organism species | Applications (RESEARCH USE ONLY!) |
| SEK504Ca | ELISA Kit for Calprotectin (CALPRO) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCK504Ca | CLIA Kit for Calprotectin (CALPRO) | Chemiluminescent immunoassay for Antigen Detection. |
| LMK504Ca | Multiplex Assay Kit for Calprotectin (CALPRO) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |