≡

Wide-range ELISA Kit for Ferritin (FE)

Specificity

This assay has high sensitivity and excellent specificity for detection of Wide-range Ferritin (FE).
No significant cross-reactivity or interference between Wide-range Ferritin (FE) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Wide-range Ferritin (FE) and the recovery rates were calculated by comparing the measured value to the expected amount of Wide-range Ferritin (FE) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 82-99 91
EDTA plasma(n=5) 90-99 94
heparin plasma(n=5) 97-105 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wide-range Ferritin (FE) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Ferritin (FE) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Wide-range Ferritin (FE) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 91-104% 91-102% 80-93% 80-90%
EDTA plasma(n=5) 86-96% 93-105% 88-97% 78-96%
heparin plasma(n=5) 84-101% 98-105% 83-102% 91-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Molecular Endocrinology PGC-1α regulates hepatic hepcidin expression and iron homeostasis in response to inflammation. Pubmed: 23438894
experimental oncology Redox-regulation of gelatinases during growth of cisplatin-sensitive and resistant Guerin carcinoma. PubMed: 25804229
J Cancer Sci Ther Host H68D Genotype Affects Tumor Growth in Mouse Melanoma Open-Access: 1948-5956-1000353
Food Funct The effects of polysaccharides from the root of Angelica sinensis on tumor growth and iron metabolism in H22-bearing mice Pubmed:26757699
Physiological research The Labile Iron Pool in Monocytes Reflects the Activity of the Atherosclerotic Process in Men with Chronic Cardiovascular Disease pubmed:27782743
Expert Review of Hematology 4 (th) Mediterranean Multidisciplinary Course on Iron Anemia April 29 (th)-30 (th) 2016, Madrid, Spain. pubmed:27615544
Oxidative Medicine and Cellular Longevity Acidic Polysaccharide from Angelica sinensis Reverses Anemia of Chronic Disease Involving the Suppression of Inflammatory Hepcidin and NF-κB Activation pubmed:29147463
European Journal of Pharmaceutical Sciences sucrosomial® iron absorption studied by in vitro and ex-vivo models pubmed:29055735
Journal of Food Science and Technology In vitro assessment of bio-augmented minerals from peanut oil cakes fermented by Aspergillus oryzae through Caco-2 cells. pubmed:29051659
International Journal of Cuban Health & Medicine Anemia and Iron Deficiency Related to Inflammation, Helicobacter pylori Infection and Adiposity in Reproductive-age Cuban Women 10.1590/MEDICC.2017.1902030004 
Oman Medical Journal The Role of Insulin Therapy in Correcting Hepcidin Levels in Patients with Type 2 Diabetes Mellitus. pubmed:28584599
Food Bioscience Preparation of lactose-iron complex and its cyto-toxicity, in-vitro digestion and bioaccessibility in Caco-2 cell model system 10.1016/j.fbio.2017.10.001
Nutrients. Long-Term Dexamethasone Exposure Down-Regulates Hepatic TFR1 and Reduces Liver Iron Concentration in Rats pubmed:28629118
Food Chemistry Physicochemical characterization of mineral (iron/zinc) bound caseinate and their mineral uptake in Caco-2 cells Pubmed:29622185
Animal Feed Science and Technology Super-dosing phytase improves the growth performance of weaner pigs fed a low iron diet 10.1016:j.anifeedsci.2018.01.012
American Journal of Hematology Iron homeostasis in pregnancy and spontaneous abortion Pubmed: 30394565
The Nigerian postgraduate medical journal the official publication of the National Postgraduate Medical College of Nigeria Assessment of iron deficiency anaemia and its risk factors among adults with chronic kidney disease in a tertiary hospital in Nigeria
Haematologica New thiazolidinones reduce iron overload in mouse models of hereditary hemochromatosis and β-thalassemia Pubmed: 30792208
博士论文 PHYSIOLOGICAL ADAPTATIONS TO HEAT ACCLIMATION; REPERCUSSIONS ON CYCLING PERFORMANCE
Synthesis, characterization and cellular mineral absorption of nanoemulsions of Rhododendron arboreum flower extracts stabilized with gum arabic
Cell Research Transferrin plays a central role in coagulation balance by interacting with clotting factors Pubmed: 31811276
international journal of biological macromolecules Gum arabic capped copper nanoparticles: Synthesis, characterization, and applications Pubmed: 31904465
journal of the american college of nutrition Improvement of Mineral Absorption and Nutritional Properties of Citrullus vulgaris Seeds Using Solid-State Fermentation Pubmed: 32255407
LWT-FOOD SCIENCE AND TECHNOLOGY Whey protein-iron or zinc complexation decreases pro-oxidant activity of iron and increases iron and zinc bioavailability
J Mol Cell Cardiol Systemic iron deficiency does not affect the cardiac iron content and progression of heart failure 34139233
European Journal of Pharmacology The mechanism of the imbalance between proliferation and ferroptosis in pulmonary artery smooth muscle cells based on the activation of SLC7A11 Pubmed:35700835
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
PAA518Ra01 Polyclonal Antibody to Ferritin (FE) WB; IHC; ICC; IP.
WEA518Ra Wide-range ELISA Kit for Ferritin (FE) Enzyme-linked immunosorbent assay for Antigen Detection.
SEA518Ra ELISA Kit for Ferritin (FE) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA518Ra Multiplex Assay Kit for Ferritin (FE) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.