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Wide-range ELISA Kit for Salusin Beta (SALb)

Specificity

This assay has high sensitivity and excellent specificity for detection of Wide-range Salusin Beta (SALb).
No significant cross-reactivity or interference between Wide-range Salusin Beta (SALb) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Wide-range Salusin Beta (SALb) and the recovery rates were calculated by comparing the measured value to the expected amount of Wide-range Salusin Beta (SALb) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 94-101 97
EDTA plasma(n=5) 88-95 92
heparin plasma(n=5) 95-103 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wide-range Salusin Beta (SALb) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Salusin Beta (SALb) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Wide-range Salusin Beta (SALb) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 95-103% 98-105% 97-105% 82-103%
EDTA plasma(n=5) 79-97% 91-103% 92-101% 94-101%
heparin plasma(n=5) 82-95% 90-104% 95-104% 85-94%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

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Magazine Citations
Journal of Maternal-Fetal and Neonatal Medicine Serum levels of apelin, salusin-alpha, and salusin-beta in normal pregnancy and preeclampsia Informa: 14767058.2012.660221
Peptides The bioactive peptides salusins and apelin–36 are produced in human arterial and venous tissues and the changes of their levels during cardiopulmonary bypass PubMed: 22884920
Department of Medical Biochemistry and Clinical Biochemistry Presence of adropin, nesfatin-1, apelin-12, ghrelins and salusins peptides in the milk, cheese whey and plasma of dairy cows ScienceDirect: S0196978113000533
European Journal of Obstetrics & Gynecology and Reproductive Biology Association of low maternal levels of salusins with gestational diabetes mellitus and with small-for-gestational-age fetuses. PubMed: 23178004
Biotech Histochem. Serum concentration and kidney expression of salusin-α and salusin-β in rats with metabolic syndrome induced by fructose. PubMed: 23244237
Gynecological Endocrinology, Salusins, newly identified regulators of hemodynamics and mitogenesis, increase in polycystic ovarian syndrome. PubMed: 22827297
Peptides Concentrations of preptin, salusins and hepcidins in plasma and milk of lactating women with or without gestational diabetes mellitus ScienceDirect: S0196978113003100
Biotechnic &amp; Histochemistry Salusin-alpha and -beta expression in heart and aorta with and without metabolic syndrome Com:Source
Med Sci Monit. Brain, Liver, and Serum Salusin-alpha and-beta Alterations in Sprague-Dawley Rats with or Without Metabolic Syndrome Pubmed:Pmc4136931
Acta Physiologica Superoxide anions involved in sympathoexcitation and pressor effects of salusin-β in paraventricular nucleus in hypertensive rats Pubmed:24304512
Pediatr Nephrol. Is the serum level of salusin-β associated with hypertension and atherosclerosis in the pediatric population? Pubmed:25245503
European Journal of Dermatology Serum salusin-α and salusin-β levels in patients with psoriasis PubMed: 26055619
J Investig Med Serum Salusin-β Levels Are Associated With the Presence and Severity of Coronary Artery Disease PubMed: 25730454
Genet Test Mol Biomarkers.  Serum Salusin-β Levels Are Correlated with Slow Coronary Flow. Pubmed:27227272
Sci Rep.  Silencing salusin-β attenuates cardiovascular remodeling and hypertension in spontaneously hypertensive rats. pubmed:28230187
Cell Death Dis. Salusin-β contributes to oxidative stress and inflammation in diabetic cardiomyopathy pubmed:28333148
Oxidative Medicine and Cellular Longevity Salusin-β Is Involved in Diabetes Mellitus-Induced Endothelial Dysfunction via Degradation of Peroxisome Proliferator-Activated Receptor Gamma 10.1155:2017
International Journal Of Cardiology Silencing salusin β ameliorates heart failure in aged spontaneously hypertensive rats by ROS-relative MAPK/NF-κB pathways in the paraventricular nucleus Pubmed: 30581101
Multiple Sclerosis and Related Disorders Increased level of Plasma Salusin-α and Salusin-β in patients with Multiple Sclerosis Pubmed: 30738876
cardiology in the young Salusin-α levels are negatively correlated with diastolic blood pressure in children with obesity Pubmed: 31486354
Redox Biology Salusin-β mediates tubular cell apoptosis in acute kidney injury: Involvement of the PKC/ROS signaling pathway Pubmed: 31884071
Journal of international medical research Salusin-β is superior to salusin-α as a marker for evaluating coronary atherosclerosis Pubmed: 32054363
cardiovascular drugs and therapy A TOR2A Gene Product: Salusin-β Contributes to Attenuated Vasodilatation of Spontaneously Hypertensive Rats. Pubmed: 32458319
Serum level of salusin β as an indicator of metabolic disorders in Acute Lymphoblastic Leucaemia and Wilms Tumour survivors
Mol Med Rep Salusin‑β participates in high glucose‑induced HK‑2 cell ferroptosis in a Nrf‑2‑dependent manner 34296310
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