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Wide-range ELISA Kit for Glia Derived Nexin (GDN)
SERPINE2; PI7; PN1; PNI; Protease Nexin I; Serpin Peptidase Inhibitor Clade E Member 2; Peptidase inhibitor 7; Protease nexin I
- Product No.WED381Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.312-20ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.127ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 466
US$ 665
US$ 2993
US$ 5653
US$ 46550
For more details, please contact local distributors!
Specificity
This assay has high sensitivity and excellent specificity for detection of Wide-range Glia Derived Nexin (GDN).
No significant cross-reactivity or interference between Wide-range Glia Derived Nexin (GDN) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Wide-range Glia Derived Nexin (GDN) and the recovery rates were calculated by comparing the measured value to the expected amount of Wide-range Glia Derived Nexin (GDN) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 89-97 | 93 |
| EDTA plasma(n=5) | 84-94 | 91 |
| heparin plasma(n=5) | 79-94 | 85 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wide-range Glia Derived Nexin (GDN) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Glia Derived Nexin (GDN) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Wide-range Glia Derived Nexin (GDN) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 94-105% | 86-93% | 91-98% | 84-105% |
| EDTA plasma(n=5) | 93-101% | 80-96% | 80-90% | 83-97% |
| heparin plasma(n=5) | 83-94% | 86-93% | 89-96% | 98-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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| Magazine | Citations |
| Medical Sciences Graduate Program | Clinical Biomarkers for the Noninvasive Diagnosis of Endometriosis handle:11375 |
| ScientificReports | The Involvement of Protease Nexin-1 (PN1) in the Pathogenesis of Intervertebral Disc (IVD) Degeneration pubmed:27460424 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPD381Hu01 | Recombinant Glia Derived Nexin (GDN) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAD381Hu01 | Polyclonal Antibody to Glia Derived Nexin (GDN) | WB; IHC; ICC; IP. |
| MAD381Hu21 | Monoclonal Antibody to Glia Derived Nexin (GDN) | WB; IHC; ICC; IP. |
| WED381Hu | Wide-range ELISA Kit for Glia Derived Nexin (GDN) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SED381Hu | ELISA Kit for Glia Derived Nexin (GDN) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMD381Hu | Multiplex Assay Kit for Glia Derived Nexin (GDN) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |