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Common Labeled Types of Antibodies, Better for Immunoassays
Antibody labeling is a common technology used in sample preparation for immunoelectron microscopy. Due to the simple operation, high sensitivity, it is widely used in various immunoassays such as IHC, WB, ICC, ELISA. The labels can amplify the detection signal for antigens, making it a fast and economic detection technology in the market. Commonly used molecules for antibody labeling include enzymes, fluorescent molecules, isotope, biotin, colloidal gold etc. Below is the introduction of conjugated antibodies from Cloud-Clone Corp. 1. Horseradish peroxidase (HRP) labeling. HRP is conjugated to monoclonal/polyclonal antibody by periodate method. The glycosyl of HRP is oxidized into al......
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The detection of the APOB family and its receptors
Human Apolipoprotein B (APOB ) was coded by APOB gene, and APOB is the primary apolipoprotein of very low-density lipoproteins(VLDL) and intermediate density lipoprotein(IDL). The levels of APOB are related to lipid metabolism, atherosclerosis and other cerebrovascular diseases. For this reason, it is the hot spot in Clinical research. While it is unclear what functional role APOB plays in low-density lipoproteins(LDL).The protein occurs in the plasma in 2 main isoforms, ApoB48 and ApoB100. The first is synthesized exclusively by the small intestine, the second by the liver. ApoB100 is the largest of the ApoB group of proteins, consisting of 4563 amino acids. In fact, ApoB48 is so call......
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Certificate of Analysis--customized labeled peptide
[ PROUDUCT ] Lot.No.: P20151130397 Protein Name: CKGGRAKDC tagged to rhodamine Size: 1mg [ QC TESTING ] Relative abundance of peptide is list bellow: Spectrum peak of labled peptide is list bellow: Relative abundance of labled peptide is list bellow:
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Principle for Immunohistochemistry (IHC) Assay
Immunohistochemistry (IHC) is also called as immunocytochemistry (ICC). It is an immunological method for qualitative and quantitative detection, as well as location of antigen in situ in cells of a tissue section, using specific and labeled antibody. Below is the principle:
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Animal Experiment can be Customized, Really?
Animal experiment is an indispensable part in many scientific fields, such as Immunology, Pharmacy, Medicine, etc.. Many researchers might list lots of headaches when mentioned animal experiment, ethical inspection is time consuming, the quality of the animal is hard to guarantee, sample collection is very complicated.... Now, there is no need to worry about these problems. Uscn Experimental Animal Inc., which is the subordinate enterprises of Cloud-clone.Corp., can provide customized animal experiment service. What kinds of service could be expected? Currently, the services include: many kinds of open animal experiments, pharmacodynamics and pharmacokinetics experiments, medicine saf......
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Quantitative Determination of Caspase-1 Expression in ATP and LPS Stimulated Monocytes by ELISA Analysis
Caspase family appears to be the key members involved in cellular apoptosis process and closely related with the morphological characteristics of apoptosis. It has been paid much attention by researchers during these years. Caspase-1 is the first discovered member, which was cloned and sequenced in 1992. After then, 14 Caspase members were cloned. Caspase-1, the key factor in the apoptosis mechanism, mainly involve in cytokine-mediated inflammatory response. Meanwhile, it plays a role in the death receptor-mediated cell apoptosis, taking part in the mature and transport of IL-1β, as well as related physiological and pathological process. During the stimulation of several factors s......