Besides for routine protein and antibody (polyclonal Ab and monoclonal Ab) custom services, based on our strong research team and served by the new bought fully automated peptidesynthesiser — aappptec Focus XC, Cloud-Clone Corp. provides new item for custom service program — Polypeptide Synthesis Service. What we can provide? Δ Quantity: More than 100 peptides per month Δ Time: About 10 days for one order Δ Length and purity: Regularly 6-25 amino acids, maximum up to 70 animo acids, with purity from 70% - 98% Δ Magnitude: From mg to g Δ Synthesizing method: Solid-phase and solution-phase synthesis Δ Labeling: FITC, HRP, Biotin la......2015-08-12

In March 2015, Cell Metabolism reported that MOTS-c, a 16-amino acid peptide encoded in the mitochondrial genome, plays an important role in metabolic homeostasis. From the research, it is found that MOTS-c mediates mitochondrial regulation of insulin through folate-purine-AMPK pathway. The discovery of MOTS-c provides new thoughts for age- and diet-dependent insulin resistance, diabetes and obesity. Moreover, the function of this peptide had been partially confirmed by mouse model (Fig.1.).The research of MOTS-c has becoming a hotspot after this initial report. In order to support the researches, we developed full suite of reagents for MOTS-c. Firstly, the peptide of MOTS-c (CPX132......2015-08-12

Small Molecules: Generally, small molecule refers to chemical compounds with low molecular weight (MW), lower than 1000 Dalton (Da). Different small molecules play distince roles in the pathology of clinical and scientific field, for instance the level of some hormones such as estron can be used as a biochemical indicator in a wide variety of infectious and noninfectious diseases. As a kind of typical hapten, small molecules lack immunogenicity which can trigger immunized animal to produce specific antibody. Thus, it is difficult for us to detect them quantified by conventional double-antibody ELISA method. However, as the development of molecule-coupling technology, plus our own modi......2015-08-12

Apoptosis, also known as programmed cell death, is a natural process or physiological death of cells in the regulation of a series of endogenous genes. Apoptosis and necrosis are two different forms of cell death, and they can be distinguised by their characteristics in morphology, biochemistry and molecular biology. There are several traditional methods of detecting apoptosis, including: 1. Apoptosis morphological examination, including observe the presence of apoptotic bodies by using optical microscope, inverted microscope, fluorescence microscope, CLSM or TEM; 2. Phosphatidylserine analysis (AnnexinV method), including using labeled Annexin-V as a fluorescent probe, detect the a......2015-08-12

Western blot (Western Blot) is a rapid and sensitive technique for detecting the protein of interest in the samples according to the antigen-antibody reactions. Western blot protocol uses gel electrophoresis (SDS-PAGE or native PAGE) to separate proteins in samples based on the difference of molecular weight of proteins . Then the proteins are transferred from the gel onto a membrane (typically nitrocellulose or PVDF), which is non-covalently bound to the proteins and can maintain the biological activity of proteins. After that, the membrane is probed using a primary antibody to detect the protein of interest followed by a further incubation with a secondary antibody conjugated to a re......2015-08-12

Elisa is a rapid, sensitive, accurate and reliable quantitative analysis method; sample preparation plays a pivotal role in a successful Elisa experiment. Collecting time, processing and storage all have influences on Elisa. Last time we introduce the common samples preparation including blood (serum, plasma), tissue homogenates, cell lysate and cell culture supernatant. This time we’ll present unusual samples such as skin, urine, feces, bronchoalveolar lavage fluid, saliva, cerebrospinal fluid, pleural effusion, prostatic fluid, semen, vaginal secretions, etc. for the reference. 1. Sputum Sputum must be treated before assaying according to the following directions. Mix the spu......2015-07-16