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Sp100 Nuclear Antigen (Sp100)

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Speckled 100 kDa; Nuclear dot-associated Sp100 protein

Sp100 Nuclear Antigen (Sp100)
CCC A326
HGNC 11206
MGI 109561
PubMed 16873258
RGD 1359550
UniProt P23497
Wiki Sp100

Sp100 nuclear antigen is an interferon stimulated antigen found in the bile duct of primary biliary cirrhosis. Histologically sp100 'dots' regions of the cell nucleus. Viral infection and mitogens affect the expression of the Sp100 autoantigen. Cells grown in the presence of interferons (α, β, and γ) revealed an increase both in size and number of the Sp100 protein-containing nuclear dots and increase the protein concentration. This raises "the question whether cytokine-mediated increase of Sp100 protein expression plays a role in induction of anti-Sp100 autoantibodies."Some sp100 variants contain a domain similar to two interferon-inducible nuclear phosphoproteins, suppressin and DEAF1. This defines a novel protein motif, the HNPP-box. Another class of variants has high mobility group 1 (HMG1) protein sequence as a domain.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA326Hu01 Recombinant Sp100 Nuclear Antigen (Sp100) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA326Hu01 Polyclonal Antibody to Sp100 Nuclear Antigen (Sp100) WB,IHC
Assay Kits n/a CLIA Kit for Sp100 Nuclear Antigen (Sp100) CLIA Kit Customized Service Offer
n/a ELISA Kit for Sp100 Nuclear Antigen (Sp100) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA326Mu01 Recombinant Sp100 Nuclear Antigen (Sp100) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA326Mu01 Polyclonal Antibody to Sp100 Nuclear Antigen (Sp100) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Sp100 Nuclear Antigen (Sp100) CLIA Kit Customized Service Offer
n/a ELISA Kit for Sp100 Nuclear Antigen (Sp100) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA326Ra01 Recombinant Sp100 Nuclear Antigen (Sp100) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA326Ra01 Polyclonal Antibody to Sp100 Nuclear Antigen (Sp100) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Sp100 Nuclear Antigen (Sp100) CLIA Kit Customized Service Offer
n/a ELISA Kit for Sp100 Nuclear Antigen (Sp100) ELISA Kit Customized Service Offer
  1. "Isolation and characterization of cDNA encoding a human nuclear antigen predominantly recognized by autoantibodies from patients with primary biliary cirrhosis."J. Immunol. 145:4338-4347(1990) [PubMed] [Europe PMC] [Abstract]
  2. "LYSP100-associated nuclear domains (LANDs): description of a new class of subnuclear structures and their relationship to PML nuclear bodies."Blood 88:1423-1426(1996) [PubMed] [Europe PMC] [Abstract]
  3. "Interaction of SP100 with HP1 proteins: a link between the promyelocytic leukemia-associated nuclear bodies and the chromatin compartment."Proc. Natl. Acad. Sci. U.S.A. 95:7316-7321(1998) [PubMed] [Europe PMC] [Abstract]
  4. "Common properties of nuclear protein SP100 and TIF1alpha chromatin factor: role of SUMO modification."Mol. Cell. Biol. 21:3314-3324(2001) [PubMed] [Europe PMC] [Abstract]
  5. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  6. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  8. "The interferon (IFN)-stimulated gene Sp100 promoter contains an IFN-gamma activation site and an imperfect IFN-stimulated response element which mediate type I IFN inducibility."J. Biol. Chem. 271:25253-25260(1996) [PubMed] [Europe PMC] [Abstract]
  9. "Splice variants of the nuclear dot-associated Sp100 protein contain homologies to HMG-1 and a human nuclear phosphoprotein-box motif."J. Cell Sci. 112:733-747(1999) [PubMed] [Europe PMC] [Abstract]
  10. "Back to the roots of a new exon-the molecular archaeology of a SP100 splice variant."Genomics 63:117-122(2000) [PubMed] [Europe PMC] [Abstract]
  11. "Molecular archeology of an SP100 splice variant revisited: dating the retrotranscription and Alu insertion events."Genome Biol. 2:RESEARCH0040.1-RESEARCH0040.6(2001) [PubMed] [Europe PMC] [Abstract]
  12. "Evidence for covalent modification of the nuclear dot-associated proteins PML and Sp100 by PIC1/SUMO-1."J. Cell Biol. 139:1621-1634(1997) [PubMed] [Europe PMC] [Abstract]
  13. "The nuclear dot protein sp100, characterization of domains necessary for dimerization, subcellular localization, and modification by small ubiquitin-like modifiers."J. Biol. Chem. 274:12555-12566(1999) [PubMed] [Europe PMC] [Abstract]
  14. "Recruitment of NBS1 into PML oncogenic domains via interaction with SP100 protein."Biochem. Biophys. Res. Commun. 299:863-871(2002) [PubMed] [Europe PMC] [Abstract]
  15. "Sp100 interacts with ETS-1 and stimulates its transcriptional activity."Mol. Cell. Biol. 22:2687-2702(2002) [PubMed] [Europe PMC] [Abstract]
  16. "Sp100 is important for the stimulatory effect of homeodomain-interacting protein kinase-2 on p53-dependent gene expression."Oncogene 22:8731-8737(2003) [PubMed] [Europe PMC] [Abstract]
  17. "SP100 expression modulates ETS1 transcriptional activity and inhibits cell invasion."Oncogene 23:6654-6665(2004) [PubMed] [Europe PMC] [Abstract]
  18. "The mammalian heterochromatin protein 1 binds diverse nuclear proteins through a common motif that targets the chromoshadow domain."Biochem. Biophys. Res. Commun. 331:929-937(2005) [PubMed] [Europe PMC] [Abstract]
  19. "Mediation of Epstein-Barr virus EBNA-LP transcriptional coactivation by Sp100."EMBO J. 24:3565-3575(2005) [PubMed] [Europe PMC] [Abstract]
  20. "Suppression of alternative lengthening of telomeres by Sp100-mediated sequestration of the MRE11/RAD50/NBS1 complex."Mol. Cell. Biol. 25:2708-2721(2005) [PubMed] [Europe PMC] [Abstract]
  21. "SP100 inhibits ETS1 activity in primary endothelial cells."Oncogene 24:916-931(2005) [PubMed] [Europe PMC] [Abstract]
  22. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  23. "FLASH links the CD95 signaling pathway to the cell nucleus and nuclear bodies."EMBO J. 26:391-401(2007) [PubMed] [Europe PMC] [Abstract]
  24. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  25. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  26. "Sp100 as a potent tumor suppressor: accelerated senescence and rapid malignant transformation of human fibroblasts through modulation of an embryonic stem cell program."Cancer Res. 70:9991-10001(2010) [PubMed] [Europe PMC] [Abstract]
  27. ErratumCancer Res. 73:4960-4961(2013) [PubMed] [Europe PMC] [Abstract]
  28. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  29. "Cdc20 mediates D-box-dependent degradation of Sp100."Biochem. Biophys. Res. Commun. 415:702-706(2011) [PubMed] [Europe PMC] [Abstract]
  30. "SP100 reduces malignancy of human glioma cells."Int. J. Oncol. 38:1023-1030(2011) [PubMed] [Europe PMC] [Abstract]
  31. "Human cytomegalovirus infection causes degradation of Sp100 proteins that suppress viral gene expression."J. Virol. 85:11928-11937(2011) [PubMed] [Europe PMC] [Abstract]
  32. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  33. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]