Packages (Simulation)

Polyclonal Antibody to Sp100 Nuclear Antigen (Sp100)

Image (I)

DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human Sp100 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Liver cancer Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human Sp100 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Breast cancer Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human Sp100 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Esophagus cancer Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human Sp100 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
AF488 staining on IF;
Sample: A431 cell
Primary Ab: 20µg/ml Rabbit Anti-Human Sp100 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Image (II)

Figure. Western Blot; Sample: Recombinant Sp100, Human.
Western Blot; Sample: Lane1: Hela cell lysate; Lane2: MCF7 cell lysate
Primary Ab: 5ug/ml Rabbit Anti-Human Sp100 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Knockout Varification:
Lane 1: Wild-type Hela cell lysate;
Lane 2: Sp100 knockout Hela cell lysate;
Predicted MW: 50,51,53,54,78,100,102kd
Observed MW: 50kd
Primary Ab: 5µg/ml Rabbit Anti-Human Sp100 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Sp100-Nuclear-Antigen-(Sp100)-PAA326Hu01.pdf

Certificate

KO-Validated

Polyclonal Antibody to Sp100 Nuclear Antigen (Sp100)

Speckled 100 kDa; Nuclear dot-associated Sp100 protein

Product No.PAA326Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPA326Hu01-Recombinant Sp100 Nuclear Antigen (Sp100)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.5mg/ml
Organism Species Moren/a
ApplicationsWB,IHC
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 94 220 US$ 220 US$ 314 US$ 785 US$ 3140
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against Sp100. It has been selected for its ability to recognize Sp100 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-5µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunofluorescence:5-20µg/mL;

Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPA326Hu01	Recombinant Sp100 Nuclear Antigen (Sp100)	Positive Control; Immunogen; SDS-PAGE; WB.
PAA326Hu01	Polyclonal Antibody to Sp100 Nuclear Antigen (Sp100)	WB,IHC