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Nitric Oxide Synthase Interacting Protein (NOSIP)

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CGI-25; eNOS-interacting protein

Nitric Oxide Synthase Interacting Protein (NOSIP)
CCC A330
HGNC 17946
MGI 1913644
PubMed 11149895
RGD 1309992
UniProt Q9Y314
Wiki NOSIP
Coimmunoprecipitation studies demonstrated the specific interaction of eNOS and NOSIP in vitro and in vivo, and complex formation was inhibited by a synthetic peptide of the caveolin-1 scaffolding domain. NO production was significantly reduced in eNOS-expressing CHO cells (CHO-eNOS) that transiently overexpressed NOSIP.
Stimulation with the calcium ionophore A23187 induced the reversible translocation of eNOS from the detergent-insoluble to the detergent-soluble fractions of CHO-eNOS, and this translocation was completely prevented by transient coexpression of NOSIP in CHO-eNOS. Immunofluorescence studies revealed a prominent plasma membrane staining for eNOS in CHO-eNOS that was abolished in the presence of NOSIP.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA330Hu01 Recombinant Nitric Oxide Synthase Interacting Protein (NOSIP) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA330Hu01 Polyclonal Antibody to Nitric Oxide Synthase Interacting Protein (NOSIP) WB,IHC
MAA330Hu21 Monoclonal Antibody to Nitric Oxide Synthase Interacting Protein (NOSIP) WB; IHC; ICC; IP.
Assay Kits SEA330Hu ELISA Kit for Nitric Oxide Synthase Interacting Protein (NOSIP) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Nitric Oxide Synthase Interacting Protein (NOSIP) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Nitric Oxide Synthase Interacting Protein (NOSIP) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Nitric Oxide Synthase Interacting Protein (NOSIP) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Nitric Oxide Synthase Interacting Protein (NOSIP) CLIA Kit Customized Service Offer
n/a ELISA Kit for Nitric Oxide Synthase Interacting Protein (NOSIP) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Nitric Oxide Synthase Interacting Protein (NOSIP) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Nitric Oxide Synthase Interacting Protein (NOSIP) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Nitric Oxide Synthase Interacting Protein (NOSIP) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Nitric Oxide Synthase Interacting Protein (NOSIP) CLIA Kit Customized Service Offer
n/a ELISA Kit for Nitric Oxide Synthase Interacting Protein (NOSIP) ELISA Kit Customized Service Offer
  1. "NOSIP, a novel modulator of endothelial nitric oxide synthase activity."FASEB J. 15:79-89(2001) [PubMed] [Europe PMC] [Abstract]
  2. "Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics."Genome Res. 10:703-713(2000) [PubMed] [Europe PMC] [Abstract]
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  4. "Nitric oxide synthase (NOS)-interacting protein interacts with neuronal NOS and regulates its distribution and activity."J. Neurosci. 24:10454-10465(2004) [PubMed] [Europe PMC] [Abstract]
  5. "Cell cycle-regulated inactivation of endothelial NO synthase through NOSIP-dependent targeting to the cytoskeleton."Mol. Cell. Biol. 25:8251-8258(2005) [PubMed] [Europe PMC] [Abstract]
  6. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
  7. "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]
  8. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  9. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  10. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  11. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  12. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]