Packages (Simulation)

Polyclonal Antibody to Nitric Oxide Synthase Interacting Protein (NOSIP)

Image (I)

DAB staining on IHC-P; Samples: Human Breast cancer Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human NOSIP Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human NOSIP Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Breast cancer Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human NOSIP Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Kidney Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human NOSIP Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Image (II)

Figure. Western Blot; Sample: Recombinant NOSIP, Human.
Knockout Varification:
Lane 1: Wild-type 293T cell lysate;
Lane 2: NOSIP knockout 293T cell lysate;
Predicted MW: 33kd
Observed MW: 35kd
Primary Ab: 5µg/ml Rabbit Anti-Human NOSIP Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human A431 cell lysate;
Primary Ab: 5µg/ml Rabbit Anti-Human NOSIP Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human Liver lysate;
Primary Ab: 5µg/ml Rabbit Anti-Human NOSIP Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human 293T cell lysate;
Primary Ab: 5µg/ml Rabbit Anti-Human NOSIP Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Nitric-Oxide-Synthase-Interacting-Protein-(NOSIP)-PAA330Hu01.pdf

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KO-Validated

Polyclonal Antibody to Nitric Oxide Synthase Interacting Protein (NOSIP)

CGI-25; eNOS-interacting protein

Product No.PAA330Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPA330Hu01-Recombinant Nitric Oxide Synthase Interacting Protein (NOSIP)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration500µg/mL
Organism Species Moren/a
ApplicationsWB,IHC
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 94 220 US$ 220 US$ 314 US$ 785 US$ 3140
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against NOSIP. It has been selected for its ability to recognize NOSIP in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-5µg/mL;
Immunohistochemistry: 5-50µg/mL;
Immunocytochemistry: 5-50µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPA330Hu01	Recombinant Nitric Oxide Synthase Interacting Protein (NOSIP)	Positive Control; Immunogen; SDS-PAGE; WB.
PAA330Hu01	Polyclonal Antibody to Nitric Oxide Synthase Interacting Protein (NOSIP)	WB,IHC
MAA330Hu21	Monoclonal Antibody to Nitric Oxide Synthase Interacting Protein (NOSIP)	WB; IHC; ICC; IP.
SEA330Hu	ELISA Kit for Nitric Oxide Synthase Interacting Protein (NOSIP)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMA330Hu	Multiplex Assay Kit for Nitric Oxide Synthase Interacting Protein (NOSIP) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.