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Midkine (MK)

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MDK; ARAP; NEGF2; Neurite Growth-Promoting Factor 2; Amphiregulin-associated protein; Midgestation and kidney protein; Neurite outgrowth-promoting factor 2

Midkine (MK)
CCC A631
HGNC 6972
MGI 96949
PubMed 20424942
RGD 69073
UniProt P21741
Wiki Midkine

Midkine, a product of a retinoic acid responsive gene, is a secreted 13 kDa protein belonging to the family of heparin binding growth/differentiation factors. MK shares 45% sequence identity with other member of this family called Pleiotrophin (HB-GAM).

Midkine is composed of two domains held together by disulfide linkages. The C-terminally located domain contains two heparin binding sites and is usually responsible for midkine activity. Part of the MK activity is enhanced by dimerization of MK. Midkine has been found in vertebrates from human to zebrafish and is most strongly expresed in midgestation. In the adult MK expression is restricted.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA631Hu01 Recombinant Midkine (MK) Positive Control; Immunogen; SDS-PAGE; WB.
RPA631Hu02 Recombinant Midkine (MK) Positive Control; Immunogen; SDS-PAGE; WB.
APA631Hu01 Active Midkine (MK) Cell culture; Activity Assays.
Antibodies PAA631Hu01 Polyclonal Antibody to Midkine (MK) WB; IHC; ICC; IP.
MAA631Hu22 Monoclonal Antibody to Midkine (MK) WB; IHC; ICC; IP.
LAA631Hu71 Biotin-Linked Polyclonal Antibody to Midkine (MK) WB; IHC; ICC.
LAA631Hu81 FITC-Linked Polyclonal Antibody to Midkine (MK) WB; IHC; ICC; IF.
PAA631Hu02 Polyclonal Antibody to Midkine (MK) WB; IHC; ICC; IP.
MAA631Hu21 Monoclonal Antibody to Midkine (MK) WB; IHC; ICC; IP.
Assay Kits SEA631Hu ELISA Kit for Midkine (MK) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA631Mu01 Recombinant Midkine (MK) Positive Control; Immunogen; SDS-PAGE; WB.
RPA631Mu02 Recombinant Midkine (MK) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA631Mu01 Polyclonal Antibody to Midkine (MK) WB; IHC; ICC; IP.
LAA631Mu71 Biotin-Linked Polyclonal Antibody to Midkine (MK) WB; IHC; ICC.
LAA631Mu81 FITC-Linked Polyclonal Antibody to Midkine (MK) WB; IHC; ICC; IF.
PAA631Mu02 Polyclonal Antibody to Midkine (MK) WB; IHC; ICC; IP.
Assay Kits SEA631Mu ELISA Kit for Midkine (MK) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA631Ra01 Recombinant Midkine (MK) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA631Ra01 Polyclonal Antibody to Midkine (MK) WB; IHC; ICC; IP.
LAA631Ra71 Biotin-Linked Polyclonal Antibody to Midkine (MK) WB; IHC; ICC.
MAA631Ra21 Monoclonal Antibody to Midkine (MK) WB; IHC; ICC; IP.
Assay Kits SEA631Ra ELISA Kit for Midkine (MK) Enzyme-linked immunosorbent assay for Antigen Detection.
  1. "A new family of heparin-binding factors: strong conservation of midkine (MK) sequences between the human and the mouse."Biochem. Biophys. Res. Commun. 176:792-797(1991) [PubMed] [Europe PMC] [Abstract]
  2. "Cloning, characterization and developmental regulation of two members of a novel human gene family of neurite outgrowth-promoting proteins."Growth Factors 5:99-114(1991) [PubMed] [Europe PMC] [Abstract]
  3. "Genomic structure of human midkine (MK), a retinoic acid-responsive growth/differentiation factor."J. Biochem. 111:563-567(1992) [PubMed] [Europe PMC] [Abstract]
  4. "Structure of the gene coding for the human retinoic acid-inducible factor, MK."DNA Cell Biol. 12:139-147(1993) [PubMed] [Europe PMC] [Abstract]
  5. "Abnormal expression, highly efficient detection and novel truncations of midkine in human tumors, cancers and cell lines."Cancer Lett. 253:60-67(2007) [PubMed] [Europe PMC] [Abstract]
  6. "Human chromosome 11 DNA sequence and analysis including novel gene identification." Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  8. "Amphiregulin-associated protein: complete amino acid sequence of a protein produced by the 12-0-tetradecanoylphorbol-13-acetate-treated human breast adenocarcinoma cell line MCF-7."Biochem. Biophys. Res. Commun. 179:572-578(1991) [PubMed] [Europe PMC] [Abstract]
  9. "Identification of novel heparin-releasable proteins, as well as the cytokines midkine and pleiotrophin, in human postheparin plasma."Arterioscler. Thromb. 13:1798-1805(1993) [PubMed] [Europe PMC] [Abstract]
  10. "Signal peptide prediction based on analysis of experimentally verified cleavage sites."Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]
  11. "Midkine binds to anaplastic lymphoma kinase (ALK) and acts as a growth factor for different cell types."J. Biol. Chem. 277:35990-35998(2002) [PubMed] [Europe PMC] [Abstract]
  12. "Solution structure of midkine, a new heparin-binding growth factor."EMBO J. 16:6936-6946(1997) [PubMed] [Europe PMC] [Abstract]