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Active Midkine (MK)

MDK; ARAP; NEGF2; Neurite Growth-Promoting Factor 2; Amphiregulin-associated protein; Midgestation and kidney protein; Neurite outgrowth-promoting factor 2

ACTIVITY TEST

Figure. The binding activity of MK with LRP1.<br/>Midkine (MK) also known as neurite growth-promoting factor 2 (NEGF2) is a protein that in humans is encoded by the MDK gene. MK is a basic heparin-binding growth factor of low molecular weight, and forms a family with pleiotrophin. It is pleiotropic, capable of exerting activities such as cell proliferation, cell migration, angiogenesis and fibrinolysis. MK may potentially be indirectly targeted as a cancer treatment as a result of its cancerous proliferation properties. Besides, Low Density Lipoprotein Receptor Related Protein 1 (LRP1) has been identified as an interactor of MK, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MK and recombinant human LRP1. Briefly, MK were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to LRP1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-MK pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of MK and LRP1 was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Magazine Citations
Midkine: From Embryogenesis to Pathogenesis and Therapy Immunochemical Methods for the Detection of Midkine in Samples of Human Origin Springer: Source
33 Midkine Controls Arteriogenesis by Regulating the Bioavailability of Vascular Endothelial Growth Factor A and the Expression of Nitric Oxide Synthase 1 and … 10.1016:j.ebiom.2017.11.020
Circulation Journal Midkine Promotes Atherosclerotic Plaque Formation Through Its Pro-Inflammatory, Angiogenic and Anti-Apoptotic Functions in Apolipoprotein E-Knockout Mice pubmed:28781288
EBioMedicine Midkine Controls Arteriogenesis by Regulating the Bioavailability of Vascular Endothelial Growth Factor A and the Expression of Nitric Oxide Synthase 1 and … Pubmed:29233575
Journal of Clinical Laboratory Analysis Clinical value of jointly detection pleural fluid Midkine, pleural fluid adenosine deaminase, and pleural fluid carbohydrate antigen 125 in the identification of nonsmall … Pubmed:29797475
Charakterisierung der funktionalen Rolle des Zytokins Midkine bei der Arteriogenese :
Scientific Reports The possible role of Dickkopf-1, Golgi protein-73 and Midkine as predictors of hepatocarcinogenesis: a review and an Egyptian study Pubmed: 32198440
Odontology Effects of different pulp-capping materials on cell death signaling pathways of lipoteichoic acid-stimulated human dental pulp stem cells Pubmed: 33206337
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA631Hu02 Recombinant Midkine (MK) Positive Control; Immunogen; SDS-PAGE; WB.
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PAA631Hu02 Polyclonal Antibody to Midkine (MK) WB; IHC; ICC; IP.
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LAA631Hu71 Biotin-Linked Polyclonal Antibody to Midkine (MK) WB; IHC; ICC.
LAA631Hu81 FITC-Linked Polyclonal Antibody to Midkine (MK) WB; IHC; ICC; IF.
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LMA631Hu Multiplex Assay Kit for Midkine (MK) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA631Hu01 ELISA Kit DIY Materials for Midkine (MK) Main materials for "Do It (ELISA Kit) Yourself".