≡

Coagulation Factor XII (F12)

[Edit]

FXII; HAF; Contact Factor; Hageman Factor

Coagulation Factor XII (F12)
CCC A677
HGNC 3530
MGI 1891012
PubMed 20022081
RGD 1359175
UniProt P00748
Wiki Factor_XII

Coagulation factor XII circulates in blood as a zymogen. This single chain zymogen is converted to a two chain serine protease with an heavy chain (alpha factor XIIa) and a light chain. The heavy chain contains two fibronectin type domains, two epidermal growth factor (EGF) like domains, a kringle domain and a proline rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta factor XIIa light chain and the alpha factor XIIa light chain becomes beta factor XIIa fraction is isolated by a two-step PEG heavy chain. Prekallikrein is cleaved by factor XII to precipitation procedure followed by form kallikrein, which then cleaves factor XII first to alpha factor XIIa and then to beta factor XIIa.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA677Hu01 Recombinant Coagulation Factor XII (F12) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA677Hu01 Polyclonal Antibody to Coagulation Factor XII (F12) WB; IHC; ICC; IP.
MAA677Hu22 Monoclonal Antibody to Coagulation Factor XII (F12) WB; IHC; ICC; IP.
Assay Kits SEA677Hu ELISA Kit for Coagulation Factor XII (F12) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA677Mu01 Recombinant Coagulation Factor XII (F12) Positive Control; Immunogen; SDS-PAGE; WB.
RPA677Mu02 Recombinant Coagulation Factor XII (F12) Positive Control; Immunogen; SDS-PAGE; WB.
EPA677Mu61 Eukaryotic Coagulation Factor XII (F12) Positive Control; Immunogen; SDS-PAGE; WB.
APA677Mu61 Active Coagulation Factor XII (F12) Cell culture; Activity Assays.
Antibodies PAA677Mu01 Polyclonal Antibody to Coagulation Factor XII (F12) WB; IHC; ICC; IP.
PAA677Mu02 Polyclonal Antibody to Coagulation Factor XII (F12) WB; IHC; ICC; IP.
Assay Kits SEA677Mu ELISA Kit for Coagulation Factor XII (F12) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA677Ra01 Recombinant Coagulation Factor XII (F12) Positive Control; Immunogen; SDS-PAGE; WB.
RPA677Ra02 Recombinant Coagulation Factor XII (F12) Positive Control; Immunogen; SDS-PAGE; WB.
RPA677Ra03 Recombinant Coagulation Factor XII (F12) Positive Control; Immunogen; SDS-PAGE; WB.
RPA677Ra04 Recombinant Coagulation Factor XII (F12) Positive Control; Immunogen; SDS-PAGE; WB.
RPA677Ra05 Recombinant Coagulation Factor XII (F12) Positive Control; Immunogen; SDS-PAGE; WB.
RPA677Ra06 Recombinant Coagulation Factor XII (F12) Positive Control; Immunogen; SDS-PAGE; WB.
RPA677Ra07 Recombinant Coagulation Factor XII (F12) Positive Control; Immunogen; SDS-PAGE; WB.
RPA677Ra08 Recombinant Coagulation Factor XII (F12) Positive Control; Immunogen; SDS-PAGE; WB.
EPA677Ra61 Eukaryotic Coagulation Factor XII (F12) Positive Control; Immunogen; SDS-PAGE; WB.
APA677Ra61 Active Coagulation Factor XII (F12) Cell culture; Activity Assays.
Antibodies PAA677Ra05 Polyclonal Antibody to Coagulation Factor XII (F12) WB; IHC; ICC; IP.
Assay Kits SEA677Ra ELISA Kit for Coagulation Factor XII (F12) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Sus scrofa; Porcine (Pig)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Coagulation Factor XII (F12) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Coagulation Factor XII (F12) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Coagulation Factor XII (F12) Polyclonal Antibody Customized Service Offer
Assay Kits SEA677Po ELISA Kit for Coagulation Factor XII (F12) Enzyme-linked immunosorbent assay for Antigen Detection.
  1. "Characterization of the human blood coagulation factor XII gene. Intron/exon gene organization and analysis of the 5'-flanking region."J. Biol. Chem. 262:13662-13673(1987) [PubMed] [Europe PMC] [Abstract]
  2. "The DNA sequence and comparative analysis of human chromosome 5." Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
  3. "cDNA sequence coding for human coagulation factor XII (Hageman)."Nucleic Acids Res. 14:3146-3146(1986) [PubMed] [Europe PMC] [Abstract]
  4. "Characterization of human blood coagulation factor XII cDNA. Prediction of the primary structure of factor XII and the tertiary structure of beta-factor XIIa."J. Biol. Chem. 260:13666-13676(1985) [PubMed] [Europe PMC] [Abstract]
  5. "Characterization of a cDNA coding for human factor XII (Hageman factor)."Biochemistry 25:1525-1528(1986) [PubMed] [Europe PMC] [Abstract]
  6. "Amino acid sequence of the heavy chain of human alpha-factor XIIa (activated Hageman factor)."J. Biol. Chem. 260:5328-5341(1985) [PubMed] [Europe PMC] [Abstract]
  7. "Amino acid sequence of human beta-factor XIIa."J. Biol. Chem. 258:10924-10933(1983) [PubMed] [Europe PMC] [Abstract]
  8. "The novel acceptor splice site mutation 11396(G-->A) in the factor XII gene causes a truncated transcript in cross-reacting material negative patients."Hum. Mol. Genet. 4:1235-1237(1995) [PubMed] [Europe PMC] [Abstract]
  9. "O-linked fucose is present in the first epidermal growth factor domain of factor XII but not protein C."J. Biol. Chem. 267:5102-5107(1992) [PubMed] [Europe PMC] [Abstract]
  10. "Screening for N-glycosylated proteins by liquid chromatography mass spectrometry."Proteomics 4:454-465(2004) [PubMed] [Europe PMC] [Abstract]
  11. "Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."J. Proteome Res. 4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract]
  12. "Factor XII gene alteration in Hageman trait detected by TaqI restriction enzyme."Blood 69:1421-1424(1987) [PubMed] [Europe PMC] [Abstract]
  13. "Histidine-rich glycoprotein binds factor XIIa with high affinity and inhibits contact-initiated coagulation."Blood 117:4134-4141(2011) [PubMed] [Europe PMC] [Abstract]
  14. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  15. "The structure of the FnI-EGF-like tandem domain of coagulation factor XII solved using SIRAS."Acta Crystallogr. F 69:94-102(2013) [PubMed] [Europe PMC] [Abstract]
  16. "Coagulation factor XII (Hageman factor) Washington D.C.: inactive factor XIIa results from Cys-571-->Ser substitution."Proc. Natl. Acad. Sci. U.S.A. 86:8319-8322(1989) [PubMed] [Europe PMC] [Abstract]
  17. "Coagulation factor XII Locarno: the functional defect is caused by the amino acid substitution Arg-353-->Pro leading to loss of a kallikrein cleavage site."Blood 84:1173-1181(1994) [PubMed] [Europe PMC] [Abstract]
  18. "Mutations in the human factor XII gene."Blood 90:3967-3977(1997) [PubMed] [Europe PMC] [Abstract]
  19. "Factor XII Tenri, a novel cross-reacting material negative factor XII deficiency, occurs through a proteasome-mediated degradation."Blood 93:4300-4308(1999) [PubMed] [Europe PMC] [Abstract]
  20. "Identification and characterization of two novel mutations (Q421K and R123P) in congenital factor XII deficiency."Thromb. Haemost. 86:1409-1415(2001) [PubMed] [Europe PMC] [Abstract]
  21. "Genetic analyses and expression studies identified a novel mutation (W486C) as a molecular basis of congenital coagulation factor XII deficiency."Blood Coagul. Fibrinolysis 15:367-373(2004) [PubMed] [Europe PMC] [Abstract]
  22. "Factor XII Shizuoka, a novel mutation (Ala392Thr) identified and characterized in a patient with congenital coagulation factor XII deficiency."Thromb. Res. 115:191-197(2005) [PubMed] [Europe PMC] [Abstract]
  23. "Missense mutations in the coagulation factor XII (Hageman factor) gene in hereditary angioedema with normal C1 inhibitor."Biochem. Biophys. Res. Commun. 343:1286-1289(2006) [PubMed] [Europe PMC] [Abstract]
  24. "Increased activity of coagulation factor XII (Hageman factor) causes hereditary angioedema type III."Am. J. Hum. Genet. 79:1098-1104(2006) [PubMed] [Europe PMC] [Abstract]