Osteopontin (OPN)
[Edit]SPP1; BNSP; BSPI; ETA1; Secreted Phosphoprotein 1; Bone Sialoprotein I; Early T-Lymphocyte Activation 1; Nephropontin; Urinary stone protein; Uropontin
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Osteopontin (OPN), is a secreted multifunctional glyco-phosphoprotein with roles in bone metabolism, immune regulation, tissue remodeling , cell survival, and tumor progression . Gene structure and chromosomal location identify OPN as a member of the small integrin-binding ligand N-linked glycoprotein (SIBLING) family that also includes BSP,BMP1,DSPP, ENAM and MEPE. Murine OPN issynthesized as a 294 amino acid (aa) precursor protein with a predicted 16 aa signal peptide and a highly unusual mature protein sequence, containing 68 acidic aa and 23 potential Ser/Thr phosphorylation sites. Although the predicted molecular weight of OPN is 31 kDa,phosphorylation and N- and O-glycosylation may allow it to appear as large as 75 kDa. Variability in post-translational modifications can influence the activity of OPN.
Organism species: Homo sapiens (Human)
| CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
| Proteins | RPA899Hu02 | Recombinant Osteopontin (OPN) | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA899Hu61 | Eukaryotic Osteopontin (OPN) | Positive Control; Immunogen; SDS-PAGE; WB. | |
| APA899Hu61 | Active Osteopontin (OPN) | Cell culture; Activity Assays. | |
| EPA899Hu62 | Eukaryotic Osteopontin (OPN) | Positive Control; Immunogen; SDS-PAGE; WB. | |
| Antibodies | MAA899Hu22 | Monoclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC; IP. |
| LAA899Hu71 | Biotin-Linked Polyclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC. | |
| PAA899Hu02 | Polyclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC; IP; FCM | |
| PAA899Hu06 | Polyclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC; IP. | |
| Assay Kits | SEA899Hu | ELISA Kit for Osteopontin (OPN) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA899Hu | CLIA Kit for Osteopontin (OPN) | Chemiluminescent immunoassay for Antigen Detection. | |
| AEA899Hu | ELISA Kit for Anti-Osteopontin Antibody (Anti-OPN) | Enzyme-linked immunosorbent assay for Antibody Detection. |
Organism species: Mus musculus (Mouse)
| CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
| Proteins | RPA899Mu01 | Recombinant Osteopontin (OPN) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA899Mu02 | Recombinant Osteopontin (OPN) | Positive Control; Immunogen; SDS-PAGE; WB. | |
| EPA899Mu61 | Eukaryotic Osteopontin (OPN) | Positive Control; Immunogen; SDS-PAGE; WB. | |
| APA899Mu61 | Active Osteopontin (OPN) | Cell culture; Activity Assays. | |
| RPA899Mu03 | Recombinant Osteopontin (OPN) | Positive Control; Immunogen; SDS-PAGE; WB. | |
| Antibodies | PAA899Mu01 | Polyclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC; IP. |
| PAA899Mu02 | Polyclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC; IP. | |
| PAA899Mu06 | Polyclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC; IP. | |
| Assay Kits | SEA899Mu | ELISA Kit for Osteopontin (OPN) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA899Mu | CLIA Kit for Osteopontin (OPN) | Chemiluminescent immunoassay for Antigen Detection. |
Organism species: Rattus norvegicus (Rat)
| CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
| Proteins | RPA899Ra01 | Recombinant Osteopontin (OPN) | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA899Ra61 | Eukaryotic Osteopontin (OPN) | Positive Control; Immunogen; SDS-PAGE; WB. | |
| APA899Ra61 | Active Osteopontin (OPN) | Cell culture; Activity Assays. | |
| Antibodies | PAA899Ra01 | Polyclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC; IP. |
| MAA899Ra21 | Monoclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC; IP. | |
| PAA899Ra06 | Polyclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC; IP. | |
| Assay Kits | SEA899Ra | ELISA Kit for Osteopontin (OPN) | Enzyme-linked immunosorbent assay for Antigen Detection. |
Organism species: Oryctolagus cuniculus (Rabbit)
| CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
| Proteins | RPA899Rb01 | Recombinant Osteopontin (OPN) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | PAA899Rb51 | Polyclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC; IP. |
| MAA899Rb21 | Monoclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC; IP. | |
| Assay Kits | SEA899Rb | ELISA Kit for Osteopontin (OPN) | Enzyme-linked immunosorbent assay for Antigen Detection. |
Organism species: Rhesus monkey (Simian)
| CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
| Proteins | RPA899Si01 | Recombinant Osteopontin (OPN) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | PAA899Si01 | Polyclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC; IP. |
| Assay Kits | SEA899Si | ELISA Kit for Osteopontin (OPN) | Enzyme-linked immunosorbent assay for Antigen Detection. |
Organism species: Canis familiaris; Canine (Dog)
| CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
| Proteins | RPA899Ca01 | Recombinant Osteopontin (OPN) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA899Ca02 | Recombinant Osteopontin (OPN) | Positive Control; Immunogen; SDS-PAGE; WB. | |
| Antibodies | PAA899Ca01 | Polyclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC; IP. |
| LAA899Ca71 | Biotin-Linked Polyclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC. | |
| Assay Kits | SEA899Ca | ELISA Kit for Osteopontin (OPN) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEA899Ca | High Sensitive ELISA Kit for Osteopontin (OPN) | Enzyme-linked immunosorbent assay for Antigen Detection. |
Organism species: Sus scrofa; Porcine (Pig)
| CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
| Proteins | RPA899Po01 | Recombinant Osteopontin (OPN) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | PAA899Po01 | Polyclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC; IP. |
| MAA899Po21 | Monoclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC; IP. | |
| MAA899Po22 | Monoclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC; IP. | |
| MAA899Po23 | Monoclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC; IP. | |
| MAA899Po24 | Monoclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC; IP. | |
| Assay Kits | SEA899Po | ELISA Kit for Osteopontin (OPN) | Enzyme-linked immunosorbent assay for Antigen Detection. |
Organism species: Bos taurus; Bovine (Cattle)
| CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
| Proteins | RPA899Bo01 | Recombinant Osteopontin (OPN) | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA899Bo61 | Eukaryotic Osteopontin (OPN) | Positive Control; Immunogen; SDS-PAGE; WB. | |
| Antibodies | PAA899Bo01 | Polyclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC; IP. |
| MAA899Bo21 | Monoclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC; IP. | |
| Assay Kits | SEA899Bo | ELISA Kit for Osteopontin (OPN) | Enzyme-linked immunosorbent assay for Antigen Detection. |
- "The cDNA and derived amino acid sequence for human osteopontin."Nucleic Acids Res. 17:3306-3306(1989) [PubMed] [Europe PMC] [Abstract]
- "cDNA cloning, mRNA distribution and heterogeneity, chromosomal location, and RFLP analysis of human osteopontin (OPN)."Genomics 7:491-502(1990) [PubMed] [Europe PMC] [Abstract]
- "Inhibition of calcium oxalate crystal growth in vitro by uropontin: another member of the aspartic acid-rich protein superfamily."Proc. Natl. Acad. Sci. U.S.A. 89:426-430(1992) [PubMed] [Europe PMC] [Abstract]
- "Genomic organization of the human osteopontin gene: exclusion of the locus from a causative role in the pathogenesis of dentinogenesis imperfecta type II."Genomics 27:155-160(1995) [PubMed] [Europe PMC] [Abstract]
- "Cloning and characterization of the human osteopontin gene and its promoter."Biochem. J. 303:255-262(1994) [PubMed] [Europe PMC] [Abstract]
- "Expression of osteopontin in human glioma. Its correlation with the malignancy."Lab. Invest. 72:55-63(1995) [PubMed] [Europe PMC] [Abstract]
- "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
- "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries." DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract]
- "Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
- "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
- "Molecular cloning and sequencing of cDNA encoding urinary stone protein, which is identical to osteopontin."Biochem. Biophys. Res. Commun. 184:859-864(1992) [PubMed] [Europe PMC] [Abstract]
- "Purification of a human milk protein closely similar to tumor-secreted phosphoproteins and osteopontin."Biochim. Biophys. Acta 996:43-48(1989) [PubMed] [Europe PMC] [Abstract]
- "Post-translationally modified residues of native human osteopontin are located in clusters: identification of 36 phosphorylation and five O-glycosylation sites and their biological implications."Biochem. J. 390:285-292(2005) [PubMed] [Europe PMC] [Abstract]
- "Phosphoproteomic analysis of the human pituitary."Pituitary 9:109-120(2006) [PubMed] [Europe PMC] [Abstract]
- "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
- "Secreted kinase phosphorylates extracellular proteins that regulate biomineralization."Science 336:1150-1153(2012) [PubMed] [Europe PMC] [Abstract]
- "LC-MS/MS characterization of O-glycosylation sites and glycan structures of human cerebrospinal fluid glycoproteins."J. Proteome Res. 12:573-584(2013) [PubMed] [Europe PMC] [Abstract]
- "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]