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Recombinant Osteopontin (OPN)

SPP1; BNSP; BSPI; ETA1; Secreted Phosphoprotein 1; Bone Sialoprotein I; Early T-Lymphocyte Activation 1; Nephropontin; Urinary stone protein; Uropontin

  • Product No.RPA899Rb01
  • Organism SpeciesOryctolagus cuniculus (Rabbit) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted
  • Predicted Molecular Mass37.4kDa
  • Accurate Molecular Mass57kDa(Analysis of differences refer to the manual)
  • Residues & TagsLeu17~Asn314 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder
  • Purity> 90%
  • Isoelectric Point4.2
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 164  US$ 410  US$ 820  US$ 2460  US$ 6150 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Journal of Biomedical Materials Research Part A Proliferation and osteogenesis of immortalized bone marrow-derived mesenchymal stem cells in porous polylactic glycolic acid scaffolds under perfusion culture PubMed: 19280635
Osteoarthritis and Cartilage Elevated osteopontin level of synovial fluid and articular cartilage is associated with disease severity in knee osteoarthritis patients PubMed: 19747583
The Journal of Rheumatology Thrombin-cleaved osteopontin levels in synovial fluid correlate with disease severity of knee osteoarthritis. Jrheum: source
International Journal of Pharmaceutics Suppression of tumor growth in xenograft model mice by small interfering RNA targeting osteopontin delivery using biocompatible poly(amino ester) ScienceDirect: S0378517312003742
European Cells and matreials The use of non-viral gene vectors for bioactive poly-(D, L-lactide) implant surfaces in bone tissue engineering Ecmjournal: Source
Journal of Equine Veterinary Science Influence of Seminal Plasma Antioxidants and Osteopontin on Fertility of the Arabian Horse ScienceDirect: Source
Basic &amp; Clinical Pharmacology &amp; Toxicology Treatment with Carnitine Enhances Bone Fracture Healing under Osteoporotic and/or Inflammatory Conditions Wiley:Source
Journal of Cellular Biochemistry Tension Force-Induced ATP Promotes Osteogenesis Through P2X7 Receptor in Osteoblasts Pubmed:24905552
Naunyn-Schmiedeberg&#039;s Archives of Pharmacology Treatment with α-lipoic acid enhances the bone healing after femoral fracture model of rats Pubmed:25038619
J Endocrinol Invest.? The interplay among iron metabolism, endothelium and inflammatory cascade in dysmetabolic disorders Pubmed:25245337
FEBS Letters Low-intensity pulsed ultrasound-induced ATP increases bone formation via the P2X7 receptor in osteoblast-like MC3T3-E1 cells Pubmed:25542352
Gene Effects of magnesium degradation products on mesenchymal stem cell fate and osteoblastogenesis PubMed: 26283150
Medicine (Baltimore) An Attempt to Evaluate Selected Aspects of “Bone–Fat Axis” Function in Healthy Individuals and Patients With Pancreatic Cancer PubMed: 26266370
Journal of Biomaterials and Tissue Engineering Osteogenic Differentiation of Canine Adipose Stem Cells Cultured in Alginate-Fibrin-Based Hydrogel Content: Asp
Primasari Osteopontin level correlates negatively with tumor shrinkage in neoadjuvant chemoradiation of locally advanced rectal cancer Index.Php: Amor
Int J Biol Sci Defective Initiation of Liver Regeneration in Osteopontin-Deficient Mice after Partial Hepatectomy due to Insufficient Activation of IL-6/Stat3 Pathway PubMed: 26327817
Journal of Kunming Medical University / Kunming Yike Daxue Xuebao Perioperative Clinical Investigation of Serum VEGF, CA19-9 and OPN Levels in Patients with Colorectal Carcinoma. Ebscohost
Am J Pathol.  Serum Osteopontin as a Novel Biomarker for Muscle Regeneration in Duchenne Muscular Dystrophy. Pubmed:26963343
circulation journal Stenotic Bicuspid and Tricuspid Aortic Valves　- Micro-Computed Tomography and Biological Indices of Calcification. pubmed:28344201
Journal of Cardiovascular Electrophysiology Effect of Cardiac Resynchronization Therapy on Myocardial Fibrosis and Relevant Cytokines in a Canine Model With Experimental Heart Failure. pubmed:28127817
American Journal of Translational Research Osteopontin stimulates matrix metalloproteinase expression through the nuclear factor-κB signaling pathway in rat temporomandibular joint and condylar chondrocytes. pubmed:28337262
Diabetes Care Targeted Microbiome Intervention by Microencapsulated Delayed-Release Niacin Beneficially Affects Insulin Sensitivity in Humans pubmed:29212824
Biomedicine & pharmacotherapy Icaritin induces MC3T3-E1 subclone14 cell differentiation through estrogen receptor-mediated ERK1/2 and p38 signaling activation. pubmed:28742995
Journal of Alzheimers Disease Urine-Based Biomarkers for Alzheimer's Disease Identified Through Coupling Computational and Experimental Methods Pubmed:30040720
Indian Journal of Nephrology Osteopontin, cardiovascular risk factors and carotid intima-Media thickness in chronic kidney disease id:233806
Carbon In Situ Self-Assembly of Graphene Oxide/Polydopamine/Sr2+ Nanosheets on Titanium Surfaces for Enhanced Osteogenic Differentiation of Mesenchymal Stem Cells Doi: 10.1016/j.carbon.2018.10.081
current eye research Detection of proteins associated with extracellular matrix regulation in the aqueous humour of patients with primary glaucoma. Pubmed: 30994369
Journal of Proteomics Identification of urinary candidate biomarkers of cisplatin-induced nephrotoxicity in patients with carcinoma Pubmed: 31629959
Acta Biomaterialia Inflammatory response to magnesium-based biodegradable implant materials Pubmed: 31610341
Journal of Clinical Medicine Calcium Kidney Stones are Associated with Increased Risk of Carotid Atherosclerosis: the Link between Urinary Stone Risks, Carotid Intima-Media Thickness, and … Pubmed: 32182704
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES Febuxostat attenuates vascular calcification induced by vitamin D3 plus nicotine in rats Pubmed: 33010420
[ENGINEERING OF BIOMATERIALS Osteoconductive potential of PLGA/bioglass composite biomaterials
Biomolecules Structural Constraint of Osteopontin Facilitates Efficient Binding to CD44 34070790
Structural Changes and Detection of Liver Fibrosis¨CRelated Protein Levels in Coculture of Alveolar Echinococcosis-Protoscoleces and Human Hepatic Stellate cells
Parasit Vectors Structural changes and expression of hepatic fibrosis-related proteins in coculture of Echinococcus multilocularis protoscoleces and human hepatic stellate … 34857049
Medicina (Kaunas) Plasma Concentrations of New Biochemical Markers of Atherosclerosis in Patients with Dyslipidemia—A Pilot Study Pubmed:35743980
Catalog No. Related products for research use of Oryctolagus cuniculus (Rabbit) Organism species Applications (RESEARCH USE ONLY!)
RPA899Rb01 Recombinant Osteopontin (OPN) Positive Control; Immunogen; SDS-PAGE; WB.
PAA899Rb51 Polyclonal Antibody to Osteopontin (OPN) WB; IHC; ICC; IP.
MAA899Rb21 Monoclonal Antibody to Osteopontin (OPN) WB; IHC; ICC; IP.
SEA899Rb ELISA Kit for Osteopontin (OPN) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA899Rb Multiplex Assay Kit for Osteopontin (OPN) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.