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Nucleolin (NCL)

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C23; Protein C23

Nucleolin (NCL)
CCC C242
HGNC 7667
MGI 97286
PubMed 22859736
RGD 3153
UniProt P19338
Wiki Nucleolin

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC242Hu01 Recombinant Nucleolin (NCL) Positive Control; Immunogen; SDS-PAGE; WB.
RPC242Hu02 Recombinant Nucleolin (NCL) Positive Control; Immunogen; SDS-PAGE; WB.
RPC242Hu03 Recombinant Nucleolin (NCL) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC242Hu01 Polyclonal Antibody to Nucleolin (NCL) WB; IHC; ICC; IP.
MAC242Hu22 Monoclonal Antibody to Nucleolin (NCL) WB; IHC; ICC; IP.
PAC242Hu02 Polyclonal Antibody to Nucleolin (NCL) WB; IHC; ICC; IP.
PAC242Hu03 Polyclonal Antibody to Nucleolin (NCL) WB; IHC; ICC; IP.
Assay Kits SEC242Hu ELISA Kit for Nucleolin (NCL) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC242Mu01 Recombinant Nucleolin (NCL) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC242Mu01 Polyclonal Antibody to Nucleolin (NCL) WB; IHC; ICC; IP.
LAC242Mu71 Biotin-Linked Polyclonal Antibody to Nucleolin (NCL) WB; IHC; ICC.
Assay Kits n/a CLIA Kit for Nucleolin (NCL) CLIA Kit Customized Service Offer
n/a ELISA Kit for Nucleolin (NCL) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Nucleolin (NCL) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Nucleolin (NCL) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Nucleolin (NCL) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Nucleolin (NCL) CLIA Kit Customized Service Offer
n/a ELISA Kit for Nucleolin (NCL) ELISA Kit Customized Service Offer
  1. "Cloning and sequencing of the human nucleolin cDNA."FEBS Lett. 250:99-105(1989) [PubMed] [Europe PMC] [Abstract]
  2. "Genomic organization and chromosomal localization of the human nucleolin gene."J. Biol. Chem. 265:14922-14931(1990) [PubMed] [Europe PMC] [Abstract]
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  4. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
  5. "Purification and partial amino acid sequencing of a fructosyllysine-specific binding protein from cell membranes of the monocyte-like cell line U937."Biochim. Biophys. Acta 1266:109-112(1995) [PubMed] [Europe PMC] [Abstract]
  6. "Major cell surface-located protein substrates of an ecto-protein kinase are homologs of known nuclear proteins."Biochemistry 33:14696-14706(1994) [PubMed] [Europe PMC] [Abstract]
  7. "Nuclear proteins that bind the pre-mRNA 3' splice site sequence r(UUAG/G) and the human telomeric DNA sequence d(TTAGGG)n."Mol. Cell. Biol. 13:4301-4310(1993) [PubMed] [Europe PMC] [Abstract]
  8. "A novel RNA polymerase II-containing complex potentiates Tat-enhanced HIV-1 transcription."EMBO J. 18:3688-3701(1999) [PubMed] [Europe PMC] [Abstract]
  9. "Functional proteomic analysis of human nucleolus."Mol. Biol. Cell 13:4100-4109(2002) [PubMed] [Europe PMC] [Abstract]
  10. "Aprataxin, a novel protein that protects against genotoxic stress."Hum. Mol. Genet. 13:1081-1093(2004) [PubMed] [Europe PMC] [Abstract]
  11. "Nucleolin interacts with telomerase."J. Biol. Chem. 279:51508-51515(2004) [PubMed] [Europe PMC] [Abstract]
  12. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  13. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
  14. "Toward a global characterization of the phosphoproteome in prostate cancer cells: identification of phosphoproteins in the LNCaP cell line."Electrophoresis 28:2027-2034(2007) [PubMed] [Europe PMC] [Abstract]
  15. "Nucleolin modulates the subcellular localization of GDNF-inducible zinc finger protein 1 and its roles in transcription and cell proliferation."Exp. Cell Res. 313:3755-3766(2007) [PubMed] [Europe PMC] [Abstract]
  16. "Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra."J. Proteome Res. 6:4150-4162(2007) [PubMed] [Europe PMC] [Abstract]
  17. "Aurora-B regulates RNA methyltransferase NSUN2."Mol. Biol. Cell 18:1107-1117(2007) [PubMed] [Europe PMC] [Abstract]
  18. "Molecular composition of IMP1 ribonucleoprotein granules."Mol. Cell. Proteomics 6:798-811(2007) [PubMed] [Europe PMC] [Abstract]
  19. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  20. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  21. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  22. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  23. "Interactions of ErbB4 and Kap1 connect the growth factor and DNA damage response pathways."Mol. Cancer Res. 8:1388-1398(2010) [PubMed] [Europe PMC] [Abstract]
  24. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  25. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  26. "Structure and function of the N-terminal nucleolin binding domain of nuclear valosin-containing protein-like 2 (NVL2) harboring a nucleolar localization signal."J. Biol. Chem. 286:21732-21741(2011) [PubMed] [Europe PMC] [Abstract]
  27. "Human senataxin resolves RNA/DNA hybrids formed at transcriptional pause sites to promote Xrn2-dependent termination."Mol. Cell 42:794-805(2011) [PubMed] [Europe PMC] [Abstract]
  28. "Splicing factor 2-associated protein p32 participates in ribosome biogenesis by regulating the binding of Nop52 and fibrillarin to preribosome particles."Mol. Cell. Proteomics 10:0-0(2011) [PubMed] [Europe PMC] [Abstract]
  29. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  30. "Nucleolar scaffold protein, WDR46, determines the granular compartmental localization of nucleolin and DDX21."Genes Cells 18:780-797(2013) [PubMed] [Europe PMC] [Abstract]
  31. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  32. "Solution structure of the RRM_1 domain of NCL protein."Submitted (JUN-2006) to the PDB data bank