Packages (Simulation)

Image (I)

DAB staining on IHC-P; Samples: Mouse Kidney Tissue; Primary Ab: 20μg/ml Rabbit Anti-Mouse NCL Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Mouse Cerebellum Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Mouse NCL Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Mouse Uterus Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Mouse NCL Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Mouse Stomach Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Mouse NCL Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Mouse Spinal cord Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Mouse NCL Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Mouse Liver Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Mouse NCL Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Uterus Tissue
Primary Ab: 20µg/ml Rabbit Anti-Mouse NCL Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Cerebellum Tissue
Primary Ab: 20µg/ml Rabbit Anti-Mouse NCL Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Sample: Mouse Kidney Tissue
Primary Ab: 20µg/ml Rabbit Anti-Mouse NCL Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Image (II)

Figure. Western Blot; Sample: Recombinant NCL, Mouse.
Figure. Western Blot; Sample: Lane1: Mouse Brain Tissue; Lane2: Mouse Spleen Tissue.

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Instruction Manual

Polyclonal-Antibody-to-Nucleolin-(NCL)-PAC242Mu01.pdf

Certificate

Polyclonal Antibody to Nucleolin (NCL)

C23; Protein C23

Product No.PAC242Mu01
Organism SpeciesMus musculus (Mouse) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPC242Mu01-Recombinant Nucleolin (NCL)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration500µg/mL
Organism Species MoreHomo sapiens (Human), Rattus norvegicus (Rat)
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 108 252 US$ 252 US$ 360 US$ 900 US$ 3600
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against NCL. It has been selected for its ability to recognize NCL in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Magazine	Citations
European Journal of Pharmaceutics and Biopharmaceutics	RNA G-quadruplex as supramolecular carrier for cancer-selective delivery
Mol Pharm	Drug Loading and Functional Efficacy of Cow, Buffalo, and Goat Milk-Derived Exosomes: A Comparative Study Pubmed:35195427
Frontiers in Physiology	Fine Tuning Inclusion in 2 of′-O-Methyl Phosphorothioate Oligonucleotides Contributes to Specific Cell Targeting for Splice-Switching Modulation Pubmed:34721051

Catalog No.	Related products for research use of Mus musculus (Mouse) Organism species	Applications (RESEARCH USE ONLY!)
RPC242Mu01	Recombinant Nucleolin (NCL)	Positive Control; Immunogen; SDS-PAGE; WB.
PAC242Mu01	Polyclonal Antibody to Nucleolin (NCL)	WB; IHC; ICC; IP.
LAC242Mu71	Biotin-Linked Polyclonal Antibody to Nucleolin (NCL)	WB; IHC; ICC.