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PIF1 5'-To-3' DNA Helicase Homolog (PIF1)

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C15orf20; ATP-dependent DNA helicase PIF1; DNA repair and recombination helicase PIF1; PIF1/RRM3 DNA helicase-like protein

PIF1 5'-To-3' DNA Helicase Homolog (PIF1)
CCC N100
HGNC 26220
MGI 2143057
PubMed 24025768
UniProt Q9H611
PIF1 is a 5-prime-to-3-prime DNA helicase that negatively regulates telomerase, a reverse transcriptase that maintains telomere length.
PIF1 reduced telomerase processivity and unwound telomeric DNA/telomerase RNA (TERC) duplexes in vitro, and it preferentially bound telomeric DNA in vitro and in vivo. PIF1 had a short half life, and its degradation was mediated by ubiquitination and proteasomal degradation. Abundance of PIF1 decreased following overexpression of an activator of the anaphase-promoting complex/cyclosome. PIF1 interacted with the catalytic telomerase subunit, TERT, and telomerase activity immunoprecipitated with PIF1 from human cell lines.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant PIF1 5'-To-3' DNA Helicase Homolog (PIF1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to PIF1 5'-To-3' DNA Helicase Homolog (PIF1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to PIF1 5'-To-3' DNA Helicase Homolog (PIF1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for PIF1 5'-To-3' DNA Helicase Homolog (PIF1) CLIA Kit Customized Service Offer
n/a ELISA Kit for PIF1 5'-To-3' DNA Helicase Homolog (PIF1) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant PIF1 5'-To-3' DNA Helicase Homolog (PIF1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to PIF1 5'-To-3' DNA Helicase Homolog (PIF1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to PIF1 5'-To-3' DNA Helicase Homolog (PIF1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for PIF1 5'-To-3' DNA Helicase Homolog (PIF1) CLIA Kit Customized Service Offer
n/a ELISA Kit for PIF1 5'-To-3' DNA Helicase Homolog (PIF1) ELISA Kit Customized Service Offer
  1. "The human Pif1 helicase, a potential Escherichia coli RecD homologue, inhibits telomerase activity."Nucleic Acids Res. 34:1393-1404(2006) [PubMed] [Europe PMC] [Abstract]
  2. "Biochemical analysis of human PIF1 helicase and functions of its N-terminal domain."Nucleic Acids Res. 36:6295-6308(2008) [PubMed] [Europe PMC] [Abstract]
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  5. "Human PIF helicase is cell cycle regulated and associates with telomerase."Cell Cycle 5:2796-2804(2006) [PubMed] [Europe PMC] [Abstract]
  6. "Mitochondrial and nuclear localization of human Pif1 helicase."Biol. Pharm. Bull. 30:1685-1692(2007) [PubMed] [Europe PMC] [Abstract]
  7. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  8. "Human Pif1 helicase unwinds synthetic DNA structures resembling stalled DNA replication forks."Nucleic Acids Res. 37:6491-6502(2009) [PubMed] [Europe PMC] [Abstract]
  9. "Human Pif1 helicase is a G-quadruplex DNA-binding protein with G-quadruplex DNA-unwinding activity."Biochem. J. 430:119-128(2010) [PubMed] [Europe PMC] [Abstract]
  10. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  11. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  12. "Pif1 family helicases suppress genome instability at G-quadruplex motifs."Nature 497:458-462(2013) [PubMed] [Europe PMC] [Abstract]
  13. "Alternative translation initiation augments the human mitochondrial proteome."Nucleic Acids Res. 41:2354-2369(2013) [PubMed] [Europe PMC] [Abstract]