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Active Angiopoietin 2 (ANGPT2)

ANG2; AGPT2

ACTIVITY TEST

Figure 1. The binding activity of ANGPT2 withTie2.
Angiopoietin is part of a family of vascular growth factors that play a role in embryonic and postnatal angiogenesis. Angiopoietin cytokines are involved with controlling microvascular permeability, vasodilation, and vasoconstriction by signaling smooth muscle cells surrounding vessels. There are now four identified angiopoietins: ANGPT1, ANGPT2, ANGPT3, ANGPT4. Angiopoietin 2 (ANGPT2) promotes cell death and disrupts vascularization. Yet, when it is in conjunction with vascular endothelial growth factors, or VEGF, it can promote neo-vascularization. Besides, TEK Tyrosine Kinase (Tie2) has been identified as an interactor of ANGPT2, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ANGPT2 and recombinant humanTie2. Briefly, ANGPT2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to Tie2-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-ANGPT2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of
ANGPT2 and Tie2 was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Microvascular Research Mutant hypoxia inducible factor-1α improves angiogenesis and tissue perfusion in ischemic rabbit skeletal muscle PubMed: 20937289
Theriogenology Evaluation of Changes in Doppler Ultrasonography Indices and Levels of Maternal Serum Angiogenic Factors throughout Pregnancy in Ewes Science: Article
Pediatr Infect Dis J Important of Angiopoietic System in Evaluation of Endothelial Damage in Children with Crimean–Congo Hemorrhagic Fever PubMed: 25831422
Oncotarget Long noncoding RNA hotair mediated angiogenesis in nasopharyngeal carcinoma by direct and indirect signaling pathways Pubmed:26717040
Experimental Animals Ginsenoside Rb1 improves cardiac function and remodeling in heart failure. pubmed:28367863
Sci Rep.  Flunarizine suppresses endothelial Angiopoietin-2 in a calcium - dependent fashion in sepsis. pubmed:28276491
PeerJ  The ratio of serum Angiopoietin-1 to Angiopoietin-2 in patients with cervical cancer is a valuable diagnostic and prognostic biomarker pubmed:28584715
Cellular Physiology and Biochemistry Angiotensin-(1-7) in Paraventricular Nucleus Contributes to the Enhanced Cardiac Sympathetic Afferent Reflex and Sympathetic Activity in Chronic Heart Failure Rats pubmed:28848201
Tumor Biology Cervical cancer cell–derived angiopoietins promote tumor progression pubmed:28720059
Anesthesiology Vasculotide, an angiopoietin-1 mimetic, restores microcirculatory perfusion and microvascular leakage and decreases fluid resuscitation requirements in … Pubmed:28968277
British Journal of Anaesthesia Vasculotide, an angiopoietin-1 mimetic, reduces pulmonary vascular leakage and preserves microcirculatory perfusion during cardiopulmonary bypass in rats 10.1016:j.bja.2018.05.049
Anticancer Research Oligodendrocytes Up-regulate the Invasive Activity of Glioblastoma Cells via the Angiopoietin-2 Signaling Pathway Pubmed: 30711932
Comparative Medicine Characterization of a Porcine Model for Von Willebrand Disease Type 1 and 3 Regarding Expression of Angiogenic Mediators in the Nonpregnant Female … Pubmed: 31526432
BMC Endocrine Disorders The role of the adipocytokines vaspin and visfatin in vascular endothelial function and insulin resistance in obese children Pubmed: 31771561
Biology of Reproduction Expression of angiogenic factors in the uteroplacental unit is altered at time of placentation in a porcine model of von Willebrand disease type 1 Pubmed: 31806575
Scientific Reports Association between Upper-airway Surgery and Ameliorative Risk Markers of endothelial function in obstructive Sleep Apnea Pubmed: 31882827
MEDIATORS OF INFLAMMATION Mica Can Alleviate TNBS-Induced Colitis in Mice by Reducing Angiotensin II and IL-17A and Increasing Angiotensin-Converting Enzyme 2, Angiotensin 1-7 … Pubmed: 33100902
Scientific Reports Integrated metabolomics and gut microbiome to the effects and mechanisms of naoxintong capsule on type 2 diabetes in rats Pubmed: 32616735
Intensive Care Med Exp The effect of targeting Tie2 on hemorrhagic shock-induced renal perfusion disturbances in rats 33997943
Gynecol Endocrinol Potential of thrombospondin-1 in treatment of polycystic ovary syndrome rat model: a preliminary study 34282706
Clinical and Experimental Immunology Clinical significance of differential serum-signatures for early prediction of severe dengue among Eastern Indian patients Pubmed:35348620
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA009Hu03 Recombinant Angiopoietin 2 (ANGPT2) Positive Control; Immunogen; SDS-PAGE; WB.
RPA009Hu01 Recombinant Angiopoietin 2 (ANGPT2) Positive Control; Immunogen; SDS-PAGE; WB.
RPA009Hu02 Recombinant Angiopoietin 2 (ANGPT2) Positive Control; Immunogen; SDS-PAGE; WB.
APA009Hu01 Active Angiopoietin 2 (ANGPT2) Cell culture; Activity Assays.
PAA009Hu02 Polyclonal Antibody to Angiopoietin 2 (ANGPT2) WB
PAA009Hu01 Polyclonal Antibody to Angiopoietin 2 (ANGPT2) WB,IHC
LAA009Hu71 Biotin-Linked Polyclonal Antibody to Angiopoietin 2 (ANGPT2) WB; IHC; ICC.
MAA009Hu22 Monoclonal Antibody to Angiopoietin 2 (ANGPT2) WB; IHC; ICC; IP.
RAA009Hu21 Recombinant Antibody to Angiopoietin 2 (ANGPT2) WB; IF; ICC; IHC; IP; FCM.
SEA009Hu ELISA Kit for Angiopoietin 2 (ANGPT2) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA009Hu CLIA Kit for Angiopoietin 2 (ANGPT2) Chemiluminescent immunoassay for Antigen Detection.
LMA009Hu Multiplex Assay Kit for Angiopoietin 2 (ANGPT2) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.