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Active Erythropoietin (EPO)

EP; Epoetin; Erythropoetin; Hematopoietin; Hemopoietin

ACTIVITY TEST

Erythropoietin (EPO) is a growth factor produced in the kidneys that stimulates the production of red blood cells. It works by promoting the division and differentiation of committed erythroid progenitors in the bone marrow. Epo additionally plays a tissue protective role in ischemia by blocking apoptosis and inducing angiogenesis. To test the effect of EPO on cell proliferation, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 8,000 cells/well with 2% serum standard 1640 which contains various concentrations of recombinant rat EPO. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 4 hours at 37 ℃. Proliferation of TF-1 cells after incubation with EPO for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 assay after incubation with recombinant rat EPO for 48h. The result was shown in Figure 2. It was obvious that EPO significantly increased cell viability of TF-1 cells. The ED50 is 3 ng/ml.

Figure 2. Cell proliferation of TF-1 cells after stimulated with recombinant rat EPO.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Magazine Citations
Endocrinology Prenatal Glucocorticoid Overexposure Causes Permanent Increases in Renal Erythropoietin Expression and Red Blood Cell Mass in the Rat Offspring Endo: source
Journal of Ethnopharmacology Inhibitory effect of polysaccharides isolated from Angelica sinensis on hepcidin expression PubMed: 21333724
PLOS ONE CD98 Positive Eosinophils Contribute to T Helper 1 Pattern Inflammation PlosOne: Source
The Journal of Clinical Endocrinology & Metabolism Alterations of Circulating Endothelial Cell and Endothelial Progenitor Cell Counts around the Ovulation PubMed: 22948762
Experimental & Molecular Medicine Long-term and stable correction of uremic anemia by intramuscular injection of plasmids containing hypoxia-regulated system of erythropoietin expression PubMed: PMC3509184
Biochem Biophys Res Commun. Reevaluation of erythropoietin production by the nephron Pubmed:24832733
Nutrition. Carbohydrate and glutamine supplementation modulates the Th1/Th2 balance after exercise performed at a simulated altitude of 4500 m. Pubmed:2528040
J Appl Physiol (1985). Decreased plasma soluble erythropoietin receptor in high-altitude excessive erythrocytosis and Chronic Mountain Sickness Pubmed:25324511
Expert Rev Proteomics Plasma hepcidin in early-stage breast cancer patients: no relationship with interleukin-6, erythropoietin and erythroferrone PubMed: 26496240
Oncotarget KIAA0101 is associated with human renal cell carcinoma proliferation and migration induced by erythropoietin pubmed:26575329
international journal of molecular sciences Expression of Iron-Related Proteins Differentiate Non-Cancerous and Cancerous Breast Tumors. pubmed:28216608
Veterinary Clinical Pathology Occult gastrointestinal bleeding is a common finding in dogs with chronic kidney disease pubmed:28186645
IOS Press Content Library An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's … articles:journal-of-alzheimers-disease
Oxidative Medicine and Cellular Longevity Acidic Polysaccharide from Angelica sinensis Reverses Anemia of Chronic Disease Involving the Suppression of Inflammatory Hepcidin and NF-κB Activation pubmed:29147463
PLoS Neglected Tropical Diseases Functional and phenotypic evaluation of eosinophils from patients with the acute form of paracoccidioidomycosis pubmed:28489854
Neurochemistry International Effects of erythropoietin on astrocytes and brain endothelial cells in primary culture during anoxia depend on simultaneous signaling by other cytokines and on duration of anoxia S0197018617304783
Pesquisa Veterinária Brasileira Relation between anaemia and bone marrow features and serum erythropoietin in dogs with chronic kidney disease 10.1590/s0100-736x2017000600011 
Medical Journal Armed Forces India Study of acute hypoxia markers in healthy subjects: Utility in post-crash investigation 10.1016/j.mjafi.2017.04.003
Neurochemistry International Effects of erythropoietin on astrocytes and brain endothelial cells in primary culture during anoxia depend on simultaneous signaling by other cytokines and on … Pubmed:29180303
Clinical and Experimental Medicine Increased NGAL level associated with iron store in chronic kidney disease with anemia Pubmed:29909502
Bulletin of Experimental Biology and Medicine Signal Mechanism of the Protective Effect of Combined Preconditioning by Amtizole and Moderate Hypoxia Pubmed:29308565
Bulletin of Experimental Biology and Medicine Expression of Hif-1α, Nf-κb, and Vegf Genes in the Liver and Blood Serum Levels of HIF-1α, Erythropoietin, VEGF, TGF-β, 8-Isoprostane, and Corticosterone in Wistar Rats with High and Low Resistance to Hypoxia. Corticosterone in … Pubmed: 30353332
FASEB JOURNAL High-altitude hypoxia decreases plasma erythropoietin soluble receptor (sEpoR) concentration in humans
Journal of Functional Foods Dietary gelatin enhances non-heme iron absorption possibly via regulation of systemic iron homeostasis in rats
博士论文 PHYSIOLOGICAL ADAPTATIONS TO HEAT ACCLIMATION; REPERCUSSIONS ON CYCLING PERFORMANCE
Nutrition Effects of carbohydrate and glutamine supplementation on cytokine production by monocytes after exercise in hypoxia: a crossover, randomized and double-blind … Pubmed: 31743809
BRITISH JOURNAL OF HAEMATOLOGY Disordered serum erythroferrone and hepcidin levels as indicators of the spontaneous abortion occurrence during early pregnancy in humans Pubmed: 32866306
JCI Insight. Increased FGF-23 levels are linked to ineffective erythropoiesis and impaired bone mineralization in myelodysplastic syndromes Pubmed: 32759495
Blood Cells, Molecules, and Diseases EPAS1 regulates proliferation of erythroblasts in chronic mountain sickness Pubmed: 32470757
Int J Endocrinol miR663 Prevents Epo Inhibition Caused by TNF-Alpha in Normoxia and Hypoxia 34367277
J Mater Sci Mater Med Loading of erythropoietin on biphasic calcium phosphate bioceramics promotes osteogenesis and angiogenesis by regulating EphB4/EphrinB2 molecules Pubmed:35072831
Effect of arsenate on erythropoietin production and autophagy induction in HepG2 cells
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