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Active Insulin Like Growth Factor Binding Protein 1 (IGFBP1)

IBP1; AFBP; IGF-BP25; PP12; Placental Protein 12; Amniotic Fluid Binding Protein; Alpha-Pregnancy-Associated Endometrial Globulin; Growth Hormone Independent-Binding Protein

ACTIVITY TEST

Insulin Like Growth Factor Binding Protein 1 (IGFBP1) is a member of the IGFBP family that binds to insulin-like growth factors (IGFs) with high affinity. IGFBP1 plays a crucial role in regulating the biological activities of IGFs, which are important for growth and development. IGFBP1 is primarily produced in the liver and is also secreted by various other tissues, including the decidua of the uterus during pregnancy. Its levels in the bloodstream are influenced by factors such as nutritional status, insulin levels, and growth hormone. To detect the activity of recombinant GFBP1,a functional ELISA assay was conducted to detect the interaction of recombinant rat IGFBP1 and recombinant human Insulin Like Growth Factor 1 (IGF1). Briefly, IGFBP1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IGF1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IGFBP1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant rat IGFBP1 and recombinant human IGF1 was shown in Figure 1, the EC50 for this effect is 0.222ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Endocrinology Lack of Dietary Carbohydrates Induces Hepatic Growth Hormone (GH) Resistance in Rats Endo: source
Endocrinology Serum IGF-I Is Not a Reliable Pharmacodynamic Marker of Exogenous Growth Hormone Activity in Mice Endo: source
Animal. Effects of a dietary crude fibre concentrate on growth in weaned piglets. pubmed:28318464
Stem Cell Research & Therapy Differentiation of human umbilical cord Wharton’s jelly-derived mesenchymal stem cells into endometrial cells pubmed:29096715
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