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Active Interleukin 1 Alpha (IL1a)

IL1-A; IL-1α; IL1F1; Preinterleukin 1 Alpha; Hematopoietin-1; Pro-Interleukin-1-Alpha; Interleukin-1 Family Member 1

ACTIVITY TEST

Interleukin 1 alpha (IL-1α) also known as hematopoietin 1 is a cytokine of the interleukin 1 family that in humans is encoded by the IL1A gene. IL-1αis produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. To test the effect of IL-1α on cell apoptosis, MCF7 cells were seeded into 96-well plates at a density of 4,000 cells/well with 5% serum standard DMEM including various concentrations of recombinant goat IL-1α. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-2 hours at 37 ℃. Proliferation of MCF7 cells after incubation with IL-1α for 72h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant goat IL-1α for 72h. The result was shown in Figure 2. It was obvious that IL-1α significantly inhibit cell viability of MCF7cells. The ED50 is 2.13 μg/ml.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Magazine Citations
The Veterinary Journal Quantitative assessment of hsp70, IL-1β and TNF-α in the spinal cord of dogs with E40K SOD1-associated degenerative myelopathy Pubmed: 24662024
Int J Clin Exp Pathol Expression of S100 family proteins in neonatal rats with sepsis and its significance PubMed: 25973048
Tumor Biology Hypoxia-induced secretion of IL-17 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma PubMed: 26695151
Parasites & Vectors Vaccination of rabbits with immunodominant antigens from Sarcoptes scabiei induced highlevels of humoral responses and pro-inflammatory cytokines but confers limited protection. pubmed:27502394
Cell Death Dis. Salusin-β contributes to oxidative stress and inflammation in diabetic cardiomyopathy pubmed:28333148
Journal of Cosmet Dermatol. Ultraviolet A photosensitivity profile of dexchlorpheniramine maleate and promethazine-based creams: Anti-inflammatory,antihistaminic, and skin barrier protection properties DOI: 10.1111/jocd.12349
Scientific Reports Optimization of Storage Temperature for Retention of Undifferentiated Cell Character of Cultured Human Epidermal Cell Sheets pubmed:28811665
Arthritis Research and Therapy Increased synovial lipodystrophy induced by high fat diet aggravates synovitis in experimental osteoarthritis. pubmed:29191221
International Journal of Molecular Sciences Screening of circular RNAs and validation of circANKRD36 associated with inflammation in patients with type 2 diabetes mellitus Pubmed:30066828
Journal of Molecular Neuroscience Neuroprotective effect of curcumin against cerebral ischemia-reperfusion via mediating autophagy and inflammation Pubmed:29243061
Journal of Maternal-Fetal & Neonatal Medicine Amniotic fluid HIF1α and exosomal HIF1α in cervical insufficiency patients with physical examination-indicated cerclage Pubmed:29357727
Brazilian Dental Journal Radiographic and Immunohistochemical Evaluation of Root Canal Treatment Using Different Irrigation Systems Pubmed: 30970054
The role of amniotic fluid exosome, hypoxia inducible fator-1α, and inflammatory cytokine in cervical insufficiency
OPEN ACCESS MACEDONIAN JOURNAL OF MEDICAL SCIENCES Seluang Fish (Rasbora Spp.) Oil Decreases Inflammatory Cytokines Via Increasing Vitamin D Level in Systemic Lupus Erythematosus
Polish Journal of Veterinary Sciences Effect of postpartum administration of ketoprofen on proinflammatory cytokine concentration and their correlation with lipogenesis and ketogenesis in Holstein … Pubmed: 31560476
Nanomedicine The toxicity of alumina nanoparticles to immune system in mice Pubmed: 32162999
Theranostics Biomechanically, structurally and functionally meticulously tailored polycaprolactone/silk fibroin scaffold for meniscus regeneration Pubmed: 32308770
STEM CELLS AND DEVELOPMENT IL-1α regulates osteogenesis and osteoclastic activity of dental follicle cells via JNK and p38 MAPK pathways Pubmed: 33107399
FASEB JOURNAL ANXA1 directs Schwann cells proliferation and migration to accelerate nerve regeneration through the FPR2/AMPK pathway Pubmed: 32908567
BIOLOGICAL TRACE ELEMENT RESEARCH Association Between Serum Selenium Level and Subclinical Mastitis in Dairy Cattle Pubmed: 32583225
Cancer Lett Photodynamic therapy induces human esophageal carcinoma cell pyroptosis by targeting the PKM2/caspase-8/caspase-3/GSDME axis 34256094
Brain Res Baicalin suppresses neuron autophagy and apoptosis by regulating astrocyte polarization in pentylenetetrazol-induced epileptic rats and PC12 cells 34780748
Animals (Basel) Inflammatory markers in uterine lavage fluids of pregnant, non-pregnant, and intrauterine device implanted mares on Days 10 and 15 post ovulation 34944269
Animals (Basel) Inflammatory Markers in Uterine Lavage Fluids of Pregnant, Non-Pregnant, and Intrauterine Device Implanted Mares on Days 10 and 15 Post Ovulation … Pubmed:34944269
Catalog No. Related products for research use of Capra hircus; Caprine (Goat) Organism species Applications (RESEARCH USE ONLY!)
APA071Cp61 Active Interleukin 1 Alpha (IL1a) Cell culture; Activity Assays.
RPA071Cp02 Recombinant Interleukin 1 Alpha (IL1a) Positive Control; Immunogen; SDS-PAGE; WB.
APA071Cp01 Active Interleukin 1 Alpha (IL1a) Cell culture; Activity Assays.
RPA071Cp01 Recombinant Interleukin 1 Alpha (IL1a) Positive Control; Immunogen; SDS-PAGE; WB.
PAA071Cp02 Polyclonal Antibody to Interleukin 1 Alpha (IL1a) WB; IHC; ICC; IP.
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LAA071Cp71 Biotin-Linked Polyclonal Antibody to Interleukin 1 Alpha (IL1a) WB; IHC; ICC.
MAA071Cp22 Monoclonal Antibody to Interleukin 1 Alpha (IL1a) WB; IHC; ICC; IP.
MAA071Cp21 Monoclonal Antibody to Interleukin 1 Alpha (IL1a) WB; IHC; ICC; IP.
MAA071Cp23 Monoclonal Antibody to Interleukin 1 Alpha (IL1a) WB; IHC; ICC; IP.
MAA071Cp24 Monoclonal Antibody to Interleukin 1 Alpha (IL1a) WB; IHC; ICC; IP.
MAA071Cp25 Monoclonal Antibody to Interleukin 1 Alpha (IL1a) WB; IHC; ICC; IP.
LAA071Cp72 Biotin-Linked Monoclonal Antibody to Interleukin 1 Alpha (IL1a) WB; IHC; ICC.
HEA071Cp High Sensitive ELISA Kit for Interleukin 1 Alpha (IL1a) Enzyme-linked immunosorbent assay for Antigen Detection.
SEA071Cp ELISA Kit for Interleukin 1 Alpha (IL1a) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA071Cp Multiplex Assay Kit for Interleukin 1 Alpha (IL1a) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
PSA071Cp01 Antibody Pair for Interleukin 1 Alpha (IL1a) ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA071Cp01 ELISA Kit DIY Materials for Interleukin 1 Alpha (IL1a) Main materials for "Do It (ELISA Kit) Yourself".