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Active Interleukin 1 Alpha (IL1a)
IL1-A; IL-1α; IL1F1; Preinterleukin 1 Alpha; Hematopoietin-1; Pro-Interleukin-1-Alpha; Interleukin-1 Family Member 1
- Product No.APA071Hu03
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 90%
- Isoelectric Point4.8
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 322
US$ 805
US$ 1610
US$ 4830
US$ 12075
For more details, please contact local distributors!
ACTIVITY TEST
IL1α(Interleukin-1 alpha) is a member of the interleukin 1 cytokine family. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces cell apoptosis. It is reported that exposure of MCF-7 cells to certain concentration of IL1α results in inhibition of cell growth. Thus, an cell proliferation assay of MCF-7 was conducted with the addition of IL1α. MCF-7 cells were seeded overnight at a density of 4,000 cells/well, and then treated with or without various concentrations of IL1α for 72h, then cells were observed by inverted microscope and cell viability was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450 nm using a microplate reader after incubating the plate for 1-4 hours in at 37 °C. Inhibition of MCF-7 cell proliferation after incubation with IL1α for 72h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with various concentrations of IL1α for 72h. The mean OD value of MCF-7 assessed by CCK-8 was shown in Figure 2. It was obvious that IL1α significantly decreased cell viability of MCF-7 cells and the EC50 was 4.69 ug/ml.
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Magazine | Citations |
| The Veterinary Journal | Quantitative assessment of hsp70, IL-1β and TNF-α in the spinal cord of dogs with E40K SOD1-associated degenerative myelopathy Pubmed: 24662024 |
| Int J Clin Exp Pathol | Expression of S100 family proteins in neonatal rats with sepsis and its significance PubMed: 25973048 |
| Tumor Biology | Hypoxia-induced secretion of IL-17 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma PubMed: 26695151 |
| Parasites & Vectors | Vaccination of rabbits with immunodominant antigens from Sarcoptes scabiei induced highlevels of humoral responses and pro-inflammatory cytokines but confers limited protection. pubmed:27502394 |
| Cell Death Dis. | Salusin-β contributes to oxidative stress and inflammation in diabetic cardiomyopathy pubmed:28333148 |
| Journal of Cosmet Dermatol. | Ultraviolet A photosensitivity profile of dexchlorpheniramine maleate and promethazine-based creams: Anti-inflammatory,antihistaminic, and skin barrier protection properties DOI: 10.1111/jocd.12349 |
| Scientific Reports | Optimization of Storage Temperature for Retention of Undifferentiated Cell Character of Cultured Human Epidermal Cell Sheets pubmed:28811665 |
| Arthritis Research and Therapy | Increased synovial lipodystrophy induced by high fat diet aggravates synovitis in experimental osteoarthritis. pubmed:29191221 |
| International Journal of Molecular Sciences | Screening of circular RNAs and validation of circANKRD36 associated with inflammation in patients with type 2 diabetes mellitus Pubmed:30066828 |
| Journal of Molecular Neuroscience | Neuroprotective effect of curcumin against cerebral ischemia-reperfusion via mediating autophagy and inflammation Pubmed:29243061 |
| Journal of Maternal-Fetal & Neonatal Medicine | Amniotic fluid HIF1α and exosomal HIF1α in cervical insufficiency patients with physical examination-indicated cerclage Pubmed:29357727 |
| Brazilian Dental Journal | Radiographic and Immunohistochemical Evaluation of Root Canal Treatment Using Different Irrigation Systems Pubmed: 30970054 |
| The role of amniotic fluid exosome, hypoxia inducible fator-1α, and inflammatory cytokine in cervical insufficiency | |
| OPEN ACCESS MACEDONIAN JOURNAL OF MEDICAL SCIENCES | Seluang Fish (Rasbora Spp.) Oil Decreases Inflammatory Cytokines Via Increasing Vitamin D Level in Systemic Lupus Erythematosus |
| Polish Journal of Veterinary Sciences | Effect of postpartum administration of ketoprofen on proinflammatory cytokine concentration and their correlation with lipogenesis and ketogenesis in Holstein … Pubmed: 31560476 |
| Nanomedicine | The toxicity of alumina nanoparticles to immune system in mice Pubmed: 32162999 |
| Theranostics | Biomechanically, structurally and functionally meticulously tailored polycaprolactone/silk fibroin scaffold for meniscus regeneration Pubmed: 32308770 |
| STEM CELLS AND DEVELOPMENT | IL-1α regulates osteogenesis and osteoclastic activity of dental follicle cells via JNK and p38 MAPK pathways Pubmed: 33107399 |
| FASEB JOURNAL | ANXA1 directs Schwann cells proliferation and migration to accelerate nerve regeneration through the FPR2/AMPK pathway Pubmed: 32908567 |
| BIOLOGICAL TRACE ELEMENT RESEARCH | Association Between Serum Selenium Level and Subclinical Mastitis in Dairy Cattle Pubmed: 32583225 |
| Cancer Lett | Photodynamic therapy induces human esophageal carcinoma cell pyroptosis by targeting the PKM2/caspase-8/caspase-3/GSDME axis 34256094 |
| Brain Res | Baicalin suppresses neuron autophagy and apoptosis by regulating astrocyte polarization in pentylenetetrazol-induced epileptic rats and PC12 cells 34780748 |
| Animals (Basel) | Inflammatory markers in uterine lavage fluids of pregnant, non-pregnant, and intrauterine device implanted mares on Days 10 and 15 post ovulation 34944269 |
| Animals (Basel) | Inflammatory Markers in Uterine Lavage Fluids of Pregnant, Non-Pregnant, and Intrauterine Device Implanted Mares on Days 10 and 15 Post Ovulation … Pubmed:34944269 |