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Active Matrix Metalloproteinase 3 (MMP3)
SL1; STMY; STMY1; STR1; Progelatinase; Stromelysin 1; Transin-1
- Product No.APA101Hu62
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 95%
- Isoelectric Point5.9
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 337
US$ 843
US$ 1686
US$ 5058
US$ 12645
For more details, please contact local distributors!
ACTIVITY TEST
Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-3 (stromelysin-1), can degrade a broad range of substrates including collagen alpha chains, aggrecan, laminin, fibronectin, and elastin. MMP-3 does not cleave the triple helical region of interstitial collagens, a characteristic which distinguishes the stromelysins from the collagenases. MMP-3 is expressed by fibroblasts, chrondrocytes, osteoblasts, endothelial cells, smooth muscle cells and macrophages. Structurally, MMP-3 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain. The activity of recombinant human MMP-3 is measured by its ability to cleave a fluorogenic peptide substrate Mca-Arg-Pro-Lys-Pro-Val-Glu-Nval-Trp-Arg-Lys(Dnp)-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. The rhMMP3 is diluted to 200 ug/ml in assay buffer, then activated with 5 ug/ml chymotrypsin at 37 ℃ for 30min followed by adding 2 mM PMSF to stop activation. The activated rhMMP3 is diluted to 6.25 ug/mL in assay buffer. Loading into a black well plate 50 µL of 6.25 ug/mL rhMMP3 and start the reaction by adding 50 µL of 20 µM substrate, with a substrate blank containing 50 µL assay buffer, 50 µL substrate, and no rhMMP3. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The specific activity of recombinant human MMP3 is > 90 pmol/min/µg.
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Magazine | Citations |
| Journal of Bone and Joint Surgery | Experimental osteoarthritis induced by surgical realignment of the patella in BALB/c mice PubMed: 21119180 |
| Infection and Immunity | Bovine Respiratory Syncytial Virus and Histophilus somni Interaction at the Alveolar Barrier Pubmed: 23649093 |
| PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source |
| China Pharmacy | Influence of Tongfengtai Granules on Inflammatory Factor in Acute Gouty Arthritis Model Rats Source |
| Thromb Haemost. | Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke Pubmed:24671655 |
| Connective Tissue Research | Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes Pubmed:25333747 |
| physiological research | The skin remodeling in type 1 diabetes and insulin resistance animal models PubMed: 26047379 |
| Physiol Res | The skin remodeling in type 1 diabetes and insulin resistance animal models. PubMed: 26047379 |
| Cellular & Molecular Immunology | Effect of ebosin on modulating interleukin-2&bgr;-induced inflammatory responses in rat fibroblast-like synoviocytes PubMed: 25938977 |
| Biol Res | The role of MCP-1-CCR2 ligand-receptor axis in chondrocyte degradation and disease progress in knee osteoarthritis PubMed: 26578310 |
| J Orthop Res. | Tetrandrine suppresses articular inflammatory response by inhibiting pro-inflammatory factors via NF-κB inactivation. Pubmed:26748661 |
| Cellular & Molecular Immunology | Effect of ebosin on modulating interleukin-1β-induced inflammatory responses in rat fibroblast-like synoviocytes pubmed:25938977 |
| Journal of Orthopaedic Research | Tetrandrine suppresses articular inflammatory response by inhibiting pro‐inflammatory factors via NF‐κB inactivation pubmed:26748661 |
| Mediators of Inflammation | Interleukin-29 Enhances Synovial Inflammation and Cartilage Degradation in Osteoarthritis. pubmed:27433031 |
| Molecular Medicine Reports | Icariin inhibits MMP‑1, MMP‑3 and MMP‑13 expression through MAPK pathways in IL‑1β‑stimulated SW1353 chondrosarcoma cells pubmed:28447732 |
| der Christian-Albrechts-Universität zu Kiel | Wirkung schonender Laserverfahren auf den Komplexaus retinalem Pigmentepithel, Bruch´scher Membranund Choroidea - mögliche Therapieoption der frühen altersabhängigen Makuladegeneration 1133074014:34 |
| REDVET - Revista electrónica de Veterinaria | Measurement of Metalloproteinase-3 levels in synovial fluid from horses as biomarker of joint disease E-ISSN: 1695-7504 |
| REDVET - Revista electrónica de Veterinaria | Measurement of Metalloproteinase-3 levels in synovial fluid from horses as biomarker of joint disease - Medición de los niveles de Metaloproteinasa-3 en liquido sinovial comobiomarcador de enfermedad articular ISSN 1695-7504 |
| Molecular Pharmacology | Dexamethasone Down-regulates Endothelin Receptors and Reduces Endothelin-induced Production of Matrix Metalloproteinases in Cultured Rat Astrocytes 10.1124/mol.116.107300 |
| Theranostics | Targeting intracellular MMPs efficiently inhibits tumor metastasis and angiogenesis Pubmed:29774078 |
| Chinese Journal of Integrative Medicine | Electroacupuncture Delays Cartilage Degeneration by Modulating Nuclear Factor-κB Signaling Pathway Pubmed: 30656598 |
| Effects of a hyperimmunized egg product on voluntary physical activity levels, serum inflammatory markers, and owner perception of joint pain of dogs with?… | |
| PLoS One | Co-culture of osteochondral explants and synovial membrane as in vitro model for osteoarthritis Pubmed: 30939166 |
| ACS Biomaterials Science & Engineering | Fluorogenic Probe for Detecting Active Matrix Metalloproteinase-3 (MMP-3) in Plasma and Peripheral Blood Neutrophils to Indicate the Severity of Rheumatoid … |
| World Journal of Pediatrics | Regulation of JAK/STAT signal pathway by miR-21 in the pathogenesis of juvenile idiopathic arthritis Pubmed: 31641939 |
| International Journal of Molecular Sciences | Matrix Metalloproteinase-3 is Key Effector of TNF-α-Induced Collagen Degradation in Skin Pubmed: 31652545 |
| JOURNAL OF CELLULAR BIOCHEMISTRY | Teneligliptin inhibits IL‐1β‐induced degradation of extracellular matrix in human chondrocytes Pubmed: 32162384 |
| Bone Research | Effects of tetrahedral framework nucleic acid/wogonin complexes on osteoarthritis Pubmed: 32047705 |
| Scientific Reports | Matrix metallopeptidase expression and modulation by transforming growth factor-β1 in equine endometrosis Pubmed: 31980722 |
| Alexandria Journal for Veterinary Sciences | The Clinicopathological Picture of Some Postpartum Disorders in Barki Ewes. |
| Identification of Polyphenolic Compounds and Hepatoprotective Activity of Artichoke (Cynara Scolymus L.) Edible Part Extracts in Rats | |
| Food Funct | Undenatured type II collagen prevents and treats the osteoarthritis and motor function degradation in T2DM patients and db/db mice 33890588 |
| journal biomedicine translational research | Peanut (Arachis hypogaea) Shells Extract and Apis dorsata Honey Reduce Matrix Metalloproteinase-3 in Monosodium Iodoacetate-Induced Osteoarthritic?¡ |
| JOURNAL OF ANIMAL SCIENCE | Physical Activity Patterns of Free Living Dogs Diagnosed with Osteoarthritis 34216471 |
| Osteoarthritis Cartilage | A systematic review on the potential value of synovial fluid biomarkers to predict clinical outcomes in cartilage repair treatments Pubmed:35618204 |
| Phytomedicine | Transcriptomic profiling revealed the role of apigenin-4′-O-α-L-rhamnoside in inhibiting the activation of rheumatoid arthritis fibroblast-like synoviocytes via MAPK … Pubmed:35660352 |
| Journal of Immunology Research | Ebosin Attenuates the Inflammatory Responses Induced by TNF-α through Inhibiting NF-κB and MAPK Pathways in Rat Fibroblast-Like Synoviocytes Pubmed:35340587 |