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ELISA Kit DIY Materials for Matrix Metalloproteinase 3 (MMP3)
SL1; STMY; STMY1; STR1; Progelatinase; Stromelysin 1; Transin-1
- Product No.KSA101Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range31.2-2000pg/mL
- Applicable Sensitivity13.1pg/mL
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- Downloadn/a
- UOM 96T*596T*10 96T*20 96T*50 96T*100
- FOB
US$ 806
US$ 1344
US$ 2419
US$ 4704
US$ 8064
For more details, please contact local distributors!
SPECIFITY
The Abs in the kit have high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 3 (MMP3). No significant cross-reactivity or interference between Matrix Metalloproteinase 3 (MMP3) and analogues was observed.
USAGE
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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| Magazine | Citations |
| Journal of Bone and Joint Surgery | Experimental osteoarthritis induced by surgical realignment of the patella in BALB/c mice PubMed: 21119180 |
| Infection and Immunity | Bovine Respiratory Syncytial Virus and Histophilus somni Interaction at the Alveolar Barrier Pubmed: 23649093 |
| PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source |
| China Pharmacy | Influence of Tongfengtai Granules on Inflammatory Factor in Acute Gouty Arthritis Model Rats Source |
| Thromb Haemost. | Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke Pubmed:24671655 |
| Connective Tissue Research | Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes Pubmed:25333747 |
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| Cellular & Molecular Immunology | Effect of ebosin on modulating interleukin-2&bgr;-induced inflammatory responses in rat fibroblast-like synoviocytes PubMed: 25938977 |
| Biol Res | The role of MCP-1-CCR2 ligand-receptor axis in chondrocyte degradation and disease progress in knee osteoarthritis PubMed: 26578310 |
| J Orthop Res. | Tetrandrine suppresses articular inflammatory response by inhibiting pro-inflammatory factors via NF-κB inactivation. Pubmed:26748661 |
| Cellular & Molecular Immunology | Effect of ebosin on modulating interleukin-1β-induced inflammatory responses in rat fibroblast-like synoviocytes pubmed:25938977 |
| Journal of Orthopaedic Research | Tetrandrine suppresses articular inflammatory response by inhibiting pro‐inflammatory factors via NF‐κB inactivation pubmed:26748661 |
| Mediators of Inflammation | Interleukin-29 Enhances Synovial Inflammation and Cartilage Degradation in Osteoarthritis. pubmed:27433031 |
| Molecular Medicine Reports | Icariin inhibits MMP‑1, MMP‑3 and MMP‑13 expression through MAPK pathways in IL‑1β‑stimulated SW1353 chondrosarcoma cells pubmed:28447732 |
| der Christian-Albrechts-Universität zu Kiel | Wirkung schonender Laserverfahren auf den Komplexaus retinalem Pigmentepithel, Bruch´scher Membranund Choroidea - mögliche Therapieoption der frühen altersabhängigen Makuladegeneration 1133074014:34 |
| REDVET - Revista electrónica de Veterinaria | Measurement of Metalloproteinase-3 levels in synovial fluid from horses as biomarker of joint disease E-ISSN: 1695-7504 |
| REDVET - Revista electrónica de Veterinaria | Measurement of Metalloproteinase-3 levels in synovial fluid from horses as biomarker of joint disease - Medición de los niveles de Metaloproteinasa-3 en liquido sinovial comobiomarcador de enfermedad articular ISSN 1695-7504 |
| Molecular Pharmacology | Dexamethasone Down-regulates Endothelin Receptors and Reduces Endothelin-induced Production of Matrix Metalloproteinases in Cultured Rat Astrocytes 10.1124/mol.116.107300 |
| Theranostics | Targeting intracellular MMPs efficiently inhibits tumor metastasis and angiogenesis Pubmed:29774078 |
| Chinese Journal of Integrative Medicine | Electroacupuncture Delays Cartilage Degeneration by Modulating Nuclear Factor-κB Signaling Pathway Pubmed: 30656598 |
| Effects of a hyperimmunized egg product on voluntary physical activity levels, serum inflammatory markers, and owner perception of joint pain of dogs with?… | |
| PLoS One | Co-culture of osteochondral explants and synovial membrane as in vitro model for osteoarthritis Pubmed: 30939166 |
| ACS Biomaterials Science & Engineering | Fluorogenic Probe for Detecting Active Matrix Metalloproteinase-3 (MMP-3) in Plasma and Peripheral Blood Neutrophils to Indicate the Severity of Rheumatoid … |
| World Journal of Pediatrics | Regulation of JAK/STAT signal pathway by miR-21 in the pathogenesis of juvenile idiopathic arthritis Pubmed: 31641939 |
| International Journal of Molecular Sciences | Matrix Metalloproteinase-3 is Key Effector of TNF-α-Induced Collagen Degradation in Skin Pubmed: 31652545 |
| JOURNAL OF CELLULAR BIOCHEMISTRY | Teneligliptin inhibits IL‐1β‐induced degradation of extracellular matrix in human chondrocytes Pubmed: 32162384 |
| Bone Research | Effects of tetrahedral framework nucleic acid/wogonin complexes on osteoarthritis Pubmed: 32047705 |
| Scientific Reports | Matrix metallopeptidase expression and modulation by transforming growth factor-β1 in equine endometrosis Pubmed: 31980722 |
| Alexandria Journal for Veterinary Sciences | The Clinicopathological Picture of Some Postpartum Disorders in Barki Ewes. |
| Identification of Polyphenolic Compounds and Hepatoprotective Activity of Artichoke (Cynara Scolymus L.) Edible Part Extracts in Rats | |
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| JOURNAL OF ANIMAL SCIENCE | Physical Activity Patterns of Free Living Dogs Diagnosed with Osteoarthritis 34216471 |
| Osteoarthritis Cartilage | A systematic review on the potential value of synovial fluid biomarkers to predict clinical outcomes in cartilage repair treatments Pubmed:35618204 |
| Phytomedicine | Transcriptomic profiling revealed the role of apigenin-4′-O-α-L-rhamnoside in inhibiting the activation of rheumatoid arthritis fibroblast-like synoviocytes via MAPK … Pubmed:35660352 |
| Journal of Immunology Research | Ebosin Attenuates the Inflammatory Responses Induced by TNF-α through Inhibiting NF-κB and MAPK Pathways in Rat Fibroblast-Like Synoviocytes Pubmed:35340587 |